Journal Article

Important role of the SDF-1/CXCR4 axis in the homing of systemically transplanted human amnion-derived mesenchymal stem cells (hAD-MSCs) to ovaries in rats with chemotherapy-induced premature ovarian insufficiency (POI)

Liu, Dandan, Hou, Jiying, Tang, Dongyuan, Ling, Li, Pan, Heng, Zeng, Qianru, Zhang, Yanqin, Fan, Ling

2022

Overview

Background Chemotherapy can induce premature ovarian insufficiency (POI). POI causes multiple sequelae and is currently incurable. As shown in our previous studies, systemically transplanted human amnion-derived mesenchymal stem cells (hAD-MSCs) home to ovaries with chemotherapy-induced POI and subsequently reduce ovarian injury and improve ovarian function in rats with POI. However, the cellular mechanisms that direct the migration and homing of hAD-MSCs to ovaries with chemotherapy-induced POI are incompletely understood. This study investigated the role of the SDF-1/CXCR4 axis in the migration and homing of systemically transplanted hAD-MSCs to ovaries with chemotherapy-induced POI and its relevant downstream signalling pathways. Methods CXCR4 expression in hAD-MSCs was assessed using Western blotting and immunofluorescence staining. hAD-MSC migration was tested using Transwell migration assays. SDF-1 levels were detected using ELISA. Seventy-two female SD rats were randomly divided into the control, POI, hAD-MSCs and hAD-MSCs + AMD3100 groups. Cyclophosphamide was used to establish rat POI models. For inhibitor treatment, hAD-MSCs were pretreated with AMD3100 before transplantation. PKH26-labeled hAD-MSCs were injected into the tail vein of POI rats 24 h after chemotherapy. After hAD-MSC transplantation, the homing of hAD-MSCs to ovaries and ovarian function and pathological changes were examined. We further investigated the molecular mechanisms by detecting the PI3K/Akt and ERK1/2 signalling pathways. Results hAD-MSCs expressed CXCR4. SDF-1 induced hAD-MSC migration in vitro. SDF-1 levels in ovaries and serum were significantly increased in rats with chemotherapy-induced POI, and ovaries with POI induced the homing of hAD-MSCs expressing CXCR4. Blocking the SDF-1/CXCR4 axis with AMD3100 significantly reduced the number of hAD-MSCs homing to ovaries with POI and further reduced their efficacy in POI treatment. The binding of SDF-1 to CXCR4 activated the PI3K/Akt signalling pathway, and LY294002 significantly inhibited hAD-MSC migration induced by SDF-1 in vitro. Moreover, inhibition of the PI3K/Akt signalling pathway significantly reduced the number of systemically transplanted hAD-MSCs homing to chemotherapy-induced ovaries in rats with POI. Conclusions SDF-1/CXCR4 axis partially mediates the migration and homing of systemically transplanted hAD-MSCs to the ovaries of rats with chemotherapy-induced POI, and the PI3K/Akt signalling pathway might be involved in the migration and homing of hAD-MSCs mediated by the SDF-1/CXCR4 axis.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Amnion - metabolism / Animals / Antineoplastic Agents / Biomedical and Life Sciences / Biomedical Engineering and Bioengineering / Cancer / Cell Biology / Cell Movement / Chemokine CXCL12 - genetics / Chemokine CXCL12 - metabolism / Chemotherapy / CXC chemokine receptor 4 (CXCR4) / Female / Human amnion-derived mesenchymal stem cells (hAD-MSCs) / Humans / Life Sciences / Medical research / Medicine, Experimental / Mesenchymal Stem Cell Transplantation / Mesenchymal Stem Cells - metabolism / Ovary - metabolism / Phosphatidylinositol 3-Kinases - metabolism / Premature ovarian insufficiency (POI) / Rats / Rats, Sprague-Dawley / Receptors, CXCR4 - genetics / Receptors, CXCR4 - metabolism / Regenerative Medicine/Tissue Engineering / Stem cell homing / Stem Cells / Stromal cell-derived factor-1 (SDF-1) / Transplantation