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Hik36–Hik43 and Rre6 act as a two-component regulatory system to control cell aggregation in Synechocystis sp. PCC6803
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Hik36–Hik43 and Rre6 act as a two-component regulatory system to control cell aggregation in Synechocystis sp. PCC6803
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Hik36–Hik43 and Rre6 act as a two-component regulatory system to control cell aggregation in Synechocystis sp. PCC6803
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Journal Article
Hik36–Hik43 and Rre6 act as a two-component regulatory system to control cell aggregation in Synechocystis sp. PCC6803
2020
Overview
In response to environmental stress the model cyanobacterium,
Synechocystis
sp. PCC6803 can switch from a planktonic state to autoaggregation and biofilm formation. The precise mechanism of this transition remains unknown. Here we investigated the role of a candidate two-component regulatory system (TCS) in controlling morphological changes, as a way to understand the intermediate molecular steps that are part of the signaling pathway. A bacterial two-hybrid assay showed that the response regulator Rre6 formed a TCS together with a split histidine kinase consisting of Hik36 and Hik43. Individual disruption mutants displayed autoaggregation in a static culture. In contrast, unlike in the wild type, high salinity did not induce biofilm formation in
Δhik36
,
Δhik43
and
Δrre6
. The expression levels of exopolysaccharide (EPS) production genes were higher in
Δhik36
and
Δhik43
, compared with the wild type, but lower in
Δrre6
, suggesting that the TCS regulated EPS production in
Synechocystis
. Rre6 interacted physically with the motor protein PilT2, that is a component of the type IV pilus system. This interaction was enhanced in a phosphomimic version of Rre6. Taken together, Hik36–Hik43–Rre6 function as an upstream component of the pili-related signal transduction cascade and control the prevention of cell adhesion and biofilm formation.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 631/326
/ 631/80
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biofilms
/ Fimbriae, Bacterial - genetics
/ Fimbriae, Bacterial - metabolism
/ Gene Expression Regulation, Bacterial - genetics
/ Gene Expression Regulation, Bacterial - physiology
/ Humanities and Social Sciences
/ Kinases
/ Pili
/ Science
