Journal Article

Chlorogenic acid and caffeine in combination inhibit fat accumulation by regulating hepatic lipid metabolism-related enzymes in mice

Zheng, Guodong, Qiu, Yangyang, Zhang, Qing-Feng, Li, Dongming

2014

Overview

Obesity has become a public health concern due to its positive association with the incidence of many diseases, and coffee components including chlorogenic acid (CGA) and caffeine have been demonstrated to play roles in the suppression of fat accumulation. To investigate the mechanism by which CGA and caffeine regulate lipid metabolism, in the present study, forty mice were randomly assigned to four groups and fed diets containing no CGA or caffeine, CGA, caffeine, or CGA+caffeine for 24 weeks. Body weight, intraperitoneal adipose tissue (IPAT) weight, and serum biochemical parameters were measured, and the activities and mRNA and protein expression of lipid metabolism-related enzymes were analysed. There was a decrease in the body weight and IPAT weight of mice fed the CGA+caffeine diet. There was a significant decrease in the serum and hepatic concentrations of total cholesterol, TAG and leptin of mice fed the CGA+caffeine diet. The activities of carnitine acyltransferase (CAT) and acyl-CoA oxidase (ACO) were increased in mice fed the caffeine and CGA+caffeine diets, while the activity of fatty acid synthase (FAS) was suppressed in those fed the CGA+caffeine diet. The mRNA expression levels of AMP-activated protein kinase (AMPK), CAT and ACO were considerably up-regulated in mice fed the CGA+caffeine diet, while those of PPARγ2 were down-regulated. The protein expression levels of AMPK were increased and those of FAS were decreased in mice fed the CGA+caffeine diet. These results indicate that CGA+caffeine suppresses fat accumulation and body weight gain by regulating the activities and mRNA and protein expression levels of hepatic lipid metabolism-related enzymes and that these effects are stronger than those exerted by CGA and caffeine individually.

• Publisher: Cambridge University Press
• Subject: Accumulation / acyl coenzyme A / Acyl-CoA Oxidase - chemistry / Acyl-CoA Oxidase - genetics / Acyl-CoA Oxidase - metabolism / Adipose tissue / Adiposity / AMP-activated protein kinase / Animals / Behaviour, Appetite and Obesity / Biological and medical sciences / blood chemistry / blood serum / Body fat / Body weight / body weight changes / Caffeine / Caffeine - therapeutic use / carnitine / Carnitine Acyltransferases - chemistry / Carnitine Acyltransferases - genetics / Carnitine Acyltransferases - metabolism / chlorogenic acid / Chlorogenic Acid - therapeutic use / cholesterol / Diet / Dietary Supplements / Enzyme Induction / Enzyme Repression / Enzymes / Fatty Acid Synthases - antagonists & inhibitors / Fatty Acid Synthases - genetics / Fatty Acid Synthases - metabolism / Fatty Liver - metabolism / Fatty Liver - pathology / Fatty Liver - prevention & control / fatty-acid synthase / Feeding. Feeding behavior / Female / Fundamental and applied biological sciences. Psychology / gene expression / Gene Expression Regulation, Enzymologic / Hyperlipidemias - prevention & control / Intra-Abdominal Fat - pathology / Intra-Abdominal Fat - secretion / leptin / Leptin - blood / Leptin - secretion / Lipid Metabolism / Liver / Liver - enzymology / Liver - metabolism / Liver - pathology / Medical sciences / messenger RNA / Mice / Mice, Inbred ICR / Neuropharmacology / obesity / Organ Size / Pharmacology. Drug treatments / Polyphenols / protein synthesis / Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer / Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) / Psychology. Psychoanalysis. Psychiatry / Psychopharmacology / Public health / Random Allocation / Rodents / Vertebrates: anatomy and physiology, studies on body, several organs or systems