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Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil
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Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil
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Journal Article
Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil
2012
Overview
An actinomycete strain designated PAL54, producing an antibacterial substance, was isolated from a Saharan soil in Ghardaïa, Algeria. Morphological and chemical studies indicated that this strain belonged to the genus
Saccharothrix
. Analysis of the 16S rDNA sequence showed a similarity level ranging between 96.9 and 99.2% within
Saccharothrix
species, with
S
.
longispora
DSM 43749
T
, the most closely related. DNA–DNA hybridization confirmed that strain PAL54 belonged to
Saccharothrix longispora
. It showed very strong activity against pathogenic Gram-positive and Gram-negative bacteria responsible for nosocomial infections and resistant to multiple antibiotics. Strain PAL54 secreted the antibiotic optimally during mid-stationary and decline phases of growth. One antibacterial compound was isolated from the culture broth and purified by HPLC. The active compound was elucidated by uv-visible and NMR spectroscopy and by mass spectrometry. The results showed that this compound was a
d
(−)-threo chloramphenicol. This is the first report of chloramphenicol production by a
Saccharothrix
species.
Publisher
Springer Netherlands,Springer Nature B.V,Springer Verlag
Subject
/ Actinomycetales - classification
/ Actinomycetales - isolation & purification
/ Actinomycetales - metabolism
/ Algeria
/ Bacteria
/ Biochemistry, Molecular Biology
/ Biomedical and Life Sciences
/ Chloramphenicol - metabolism
/ Chromatography, High Pressure Liquid
/ DNA
/ Environmental Engineering/Biotechnology
/ genetics
/ Gram-Negative Bacteria - drug effects
/ Gram-Positive Bacteria - drug effects
/ high performance liquid chromatography
/ Magnetic Resonance Spectroscopy
/ nuclear magnetic resonance spectroscopy
/ RNA, Ribosomal, 16S - genetics
/ soil
/ Spectrophotometry, Ultraviolet
/ Studies
/ Taxonomy
