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Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice
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Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice
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Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice
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Journal Article
Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice
2025
Overview
By transplanting pancreatic islets onto the dura mater of the mouse brain, we establish a microscopy platform that enables longitudinal intravital imaging of otherwise optically inaccessible tissue. The system combines a cranial window with an air-cushioned floating arena and stable head fixation, providing high mechanical stability for repeated single-cell Ca
2+
imaging sessions of up to 90 min in awake mice. We show that dura mater-engrafted islets integrate with host vascular and neural networks, and that human islet grafts secrete C-peptide in response to glucose stimulation, indicating metabolic integration. With this platform, we monitor anesthesia-induced changes in capillary blood flow and islet Ca
2+
dynamics. In awake mice, following subcutaneous glucose injection, we characterize intracellular Ca
2+
oscillations in insulin-secreting β-cells, revealing changes in amplitude, period, and plateau fraction while network coordination remains stable. The dura mater thus offers long-term optical access to functional endocrine tissue, facilitating stable intravital imaging under anesthesia-free, physiological conditions.
Imaging pancreatic islets in living animals is often limited by poor optical access and motion instability. Here, the authors show that transplanting islets onto the brain’s dura mater enables stable, long-term, and functional imaging in awake mice.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 64/110
/ 64/60
/ 96/34
/ 96/95
/ Animals
/ Brain
/ Dura Mater - diagnostic imaging
/ Glucose
/ Humanities and Social Sciences
/ Humans
/ Insulin
/ Insulin-Secreting Cells - metabolism
/ Intravital Microscopy - methods
/ Islets of Langerhans - cytology
/ Islets of Langerhans - metabolism
/ Islets of Langerhans Transplantation - methods
/ Male
/ Mice
/ Pancreatic islet transplantation
/ Science
