Asset Details
Do you wish to reserve the book?
Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Do you wish to request the book?
Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
2009
Overview
Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1. We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of \"slow-moving\" Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2-dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Antigens
/ DNA
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - physiology
/ Fungi
/ Gels
/ Mutation
/ Plasmids
/ proliferating cell nuclear antigen
/ Proliferating Cell Nuclear Antigen - metabolism
/ proliferating cell nuclear antigen-interacting protein
/ Proteins
/ Schizosaccharomyces - genetics
/ Schizosaccharomyces - metabolism
/ Schizosaccharomyces pombe Proteins - genetics
/ Schizosaccharomyces pombe Proteins - physiology
/ Sequence Homology, Amino Acid
/ site-specific replication termination
/ Small Ubiquitin-Related Modifier Proteins - metabolism
/ Two-Hybrid System Techniques
/ Yeasts
