Asset Details
Do you wish to reserve the book?
Dynamic gene expression in salivary glands of the cat flea during Rickettsia felis infection
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dynamic gene expression in salivary glands of the cat flea during Rickettsia felis infection
Do you wish to request the book?
Dynamic gene expression in salivary glands of the cat flea during Rickettsia felis infection
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dynamic gene expression in salivary glands of the cat flea during Rickettsia felis infection
2021
Overview
ABSTRACT
The cat flea, Ctenocephalides felis, is an arthropod vector capable of transmitting several human pathogens including Rickettsia species. Earlier studies identified Rickettsia felis in the salivary glands of the cat flea and transmission of rickettsiae during arthropod feeding. The saliva of hematophagous insects contains multiple biomolecules with anticlotting, vasodilatory and immunomodulatory activities. Notably, the exact role of salivary factors in the molecular interaction between flea-borne rickettsiae and their insect host is still largely unknown. To determine if R. felis modulates gene expression in the cat flea salivary glands, cat fleas were infected with R. felis and transcription patterns of selected salivary gland-derived factors, including antimicrobial peptides and flea-specific antigens, were assessed. Salivary glands were microdissected from infected and control cat fleas at different time points after exposure and total RNA was extracted and subjected to reverse-transcriptase quantitative PCR for gene expression analysis. During the experimental 10-day feeding period, a dynamic change in gene expression of immunity-related transcripts and salivary antigens between the two experimental groups was detected. The data indicated that defensin-2 (Cf-726), glycine-rich antimicrobial peptide (Cf-83), salivary antigens (Cf-169 and Cf-65) and deorphanized peptide (Cf-75) are flea-derived factors responsive to rickettsial infection.
This study demonstrated gene expression patterns of immunity-related and flea-specific transcripts in the salivary glands of the cat flea in response to Rickettsia felis infection.
Publisher
Oxford University Press
Subject
/ Antigens
/ Antiinfectives and antibacterials
/ Antimicrobial Peptides - analysis
/ Antimicrobial Peptides - genetics
/ Antimicrobial Peptides - metabolism
/ Ctenocephalides - metabolism
/ Ctenocephalides - microbiology
/ Female
/ Glycine
/ Insects
/ Male
/ Peptides
/ Rickettsia felis - metabolism
/ Rickettsia felis - pathogenicity
/ Rickettsia Infections - genetics
/ Rickettsia Infections - metabolism
/ Rickettsia Infections - microbiology
/ Saliva
/ Salivary Glands - metabolism
