Asset Details
Do you wish to reserve the book?
Limiting replication stress during somatic cell reprogramming reduces genomic instability in induced pluripotent stem cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Limiting replication stress during somatic cell reprogramming reduces genomic instability in induced pluripotent stem cells
Do you wish to request the book?
Limiting replication stress during somatic cell reprogramming reduces genomic instability in induced pluripotent stem cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Limiting replication stress during somatic cell reprogramming reduces genomic instability in induced pluripotent stem cells
2015
Overview
The generation of induced pluripotent stem cells (iPSC) from adult somatic cells is one of the most remarkable discoveries in recent decades. However, several works have reported evidence of genomic instability in iPSC, raising concerns on their biomedical use. The reasons behind the genomic instability observed in iPSC remain mostly unknown. Here we show that, similar to the phenomenon of oncogene-induced replication stress, the expression of reprogramming factors induces replication stress. Increasing the levels of the checkpoint kinase 1 (CHK1) reduces reprogramming-induced replication stress and increases the efficiency of iPSC generation. Similarly, nucleoside supplementation during reprogramming reduces the load of DNA damage and genomic rearrangements on iPSC. Our data reveal that lowering replication stress during reprogramming, genetically or chemically, provides a simple strategy to reduce genomic instability on mouse and human iPSC.
The expression of reprogramming factors can induce replication stress in induced pluripotent stem cells. In this study, to reduce such genomic instability, Ruiz
et al.
increase CHK1 kinase levels and nucleoside supplementation during reprogramming.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Pub. Group
Subject
/ 13/44
/ Animals
/ Biochemistry, Molecular Biology
/ Cell Proliferation - physiology
/ Cellular Reprogramming - physiology
/ Gene Expression Regulation - physiology
/ Genomic Instability - physiology
/ Genomics
/ Humanities and Social Sciences
/ Humans
/ Induced Pluripotent Stem Cells - physiology
/ Mice
/ Plasmids
/ Protein Kinases - metabolism
/ Science
