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Comparison of Cas9 activators in multiple species

by Housden, Benjamin E , Ter-Ovanesyan, Dmitry , Ewen-Campen, Ben , Collins, James J , Perrimon, Norbert , Chavez, Alejandro , Pruitt, Benjamin W , Tuttle, Marcelle , Haque, Sabina J , Kowal, Emma J K , Chari, Raj , Church, George , Buchthal, Joanna , Cecchi, Ryan J

in 631/1647/1511 / 631/337/572 / analysis / Animals / Bioinformatics / Biological Microscopy / Biological Techniques / Biomedical Engineering/Biotechnology / Cells, Cultured / Cellular biology / Cellular proteins / CRISPR-Associated Proteins - metabolism / Drosophila melanogaster - genetics / Drosophila melanogaster - metabolism / Gene expression / Genes, vpr / Genetic Engineering / Humans / Life Sciences / Mice / Observations / Properties / Proteins / Proteomics / Trans-Activators - metabolism / Transcription factors / Transcription Factors - genetics / Transcription Factors - metabolism

2016

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Comparison of Cas9 activators in multiple species

2016

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Comparison of Cas9 activators in multiple species

2016

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Journal Article

Comparison of Cas9 activators in multiple species

Housden, Benjamin E,

Ter-Ovanesyan, Dmitry,

Ewen-Campen, Ben,

Collins, James J,

Perrimon, Norbert,

Chavez, Alejandro,

Pruitt, Benjamin W,

Tuttle, Marcelle,

Haque, Sabina J,

Kowal, Emma J K,

Chari, Raj,

Church, George,

Buchthal, Joanna,

Cecchi, Ryan J

2016

Overview

A comparison of seven dCas9-based transcriptional activators shows that VPR, SAM, and Suntag perform best in cell lines from a variety of organisms. Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.

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Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

/ Animals

/ Biological Microscopy

/ Biological Techniques