Journal Article

Transduced Tat-DJ-1 protein protects against oxidative stress-induced SH-SY5Y cell death and Parkinson disease in a mouse model

Choi, S.Y., Hallym University, Chuncheon, Republic of Korea, Kim, S.M., Hallym University, Chuncheon, Republic of Korea, Han, K.H., Hallym University, Chuncheon, Republic of Korea, Kim, H.R., Hallym University, Chuncheon, Republic of Korea, Jo, H.S., Hallym University, Chuncheon, Republic of Korea, Eum, W.S., Hallym University, Chuncheon, Republic of Korea, Park, M.Y., Hallym University, Chuncheon, Republic of Korea, Kwon, O.S., Kyungpook National University, Daegu, Republic of Korea, Jeong, H.J., Hallym University, Chuncheon, Republic of Korea, Woo, S.J., Hallym University, Chuncheon, Republic of Korea, Hwang, I.K., Seoul National University, Seoul, Republic of Korea, Park, J.S., Hallym University, Chuncheon, Republic of Korea, Kim, D.W., Hallym University, Chuncheon, Republic of Korea, Kim, D.S., Soonchunhyang University, Cheonan, Republic of Korea

2012

Overview

Parkinson's disease (PD) is a well known neurodegenerative disorder characterized by selective loss of dopaminergic neurons in the substantia nigra pars compact (SN). Although the exact mechanism remains unclear, oxidative stress plays a critical role in the pathogenesis of PD. DJ-1 is a multifunctional protein, a potent antioxidant and chaperone, the loss of function of which is linked to the autosomal recessive early onset of PD. Therefore, we investigated the protective effects of DJ-1 protein against SH-SY5Y cells and in a PD mouse model using a cell permeable Tat-DJ-1 protein. Tat-DJ-1 protein rapidly transduced into the cells and showed a protective effect on 6-hydroxydopamine (6-OHDA)-induced neuronal cell death by reducing the reactive oxygen species (ROS). In addition, we found that Tat-DJ-1 protein protects against dopaminergic neuronal cell death in 1-methyl-4-phenyl-1,2,3,6,-tetrahydropyridine (MPTP)-induced PD mouse models. These results suggest that Tat-DJ-1 protein provides a potential therapeutic strategy for against ROS related human diseases including PD.

• Publisher: Korean Society for Molecular and Cellular Biology,Korea Society for Molecular and Cellular Biology,한국분자세포생물학회
• Subject: ANTIOXIDANTES / ANTIOXIDANTS / ANTIOXYDANT / Biochemistry / Biomedical and Life Sciences / Biomedicine / Biotechnology / Cell Biology / ESPECIES DE OXIGENO REACTIVO / Life Sciences / Parkinson disease / protein transduction / REACTIVE OXYGEN SPECIES / 생물학