Asset Details
Do you wish to reserve the book?
Dicer1 activity in the stromal compartment regulates nephron differentiation and vascular patterning during mammalian kidney organogenesis
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dicer1 activity in the stromal compartment regulates nephron differentiation and vascular patterning during mammalian kidney organogenesis
Do you wish to request the book?
Dicer1 activity in the stromal compartment regulates nephron differentiation and vascular patterning during mammalian kidney organogenesis
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dicer1 activity in the stromal compartment regulates nephron differentiation and vascular patterning during mammalian kidney organogenesis
2015
Overview
MicroRNAs, activated by the enzyme Dicer1, control post-transcriptional gene expression. Dicer1 has important roles in the epithelium during nephrogenesis, but its function in stromal cells during kidney development is unknown. To study this, we inactivated Dicer1 in renal stromal cells. This resulted in hypoplastic kidneys, abnormal differentiation of the nephron tubule and vasculature, and perinatal mortality. In mutant kidneys, genes involved in stromal cell migration and activation were suppressed as were those involved in epithelial and endothelial differentiation and maturation. Consistently, polarity of the proximal tubule was incorrect, distal tubule differentiation was diminished, and elongation of Henle’s loop attenuated resulting in lack of inner medulla and papilla in stroma-specific Dicer1 mutants. Glomerular maturation and capillary loop formation were abnormal, whereas peritubular capillaries, with enhanced branching and increased diameter, formed later. In Dicer1-null renal stromal cells, expression of factors associated with migration, proliferation, and morphogenic functions including α-smooth muscle actin, integrin-α8, -β1, and the WNT pathway transcriptional regulator LEF1 were reduced. Dicer1 mutation in stroma led to loss of expression of distinct microRNAs. Of these, miR-214, -199a-5p, and -199a-3p regulate stromal cell functions ex vivo, including WNT pathway activation, migration, and proliferation. Thus, Dicer1 activity in the renal stromal compartment regulates critical stromal cell functions that, in turn, regulate differentiation of the nephron and vasculature during nephrogenesis.
Publisher
Elsevier Inc,Elsevier Limited
Subject
/ Animals
/ Cell Differentiation - genetics
/ Cell Proliferation - genetics
/ DEAD-box RNA Helicases - genetics
/ DEAD-box RNA Helicases - metabolism
/ DEAD-box RNA Helicases - physiology
/ dicer1
/ Female
/ foxd1
/ Integrin alpha Chains - metabolism
/ Kidney Glomerulus - blood supply
/ Kidney Glomerulus - cytology
/ Kidney Glomerulus - embryology
/ Kidney Tubules - blood supply
/ Kidney Tubules, Distal - blood supply
/ Kidney Tubules, Distal - cytology
/ Kidney Tubules, Distal - embryology
/ Kidney Tubules, Proximal - blood supply
/ Kidney Tubules, Proximal - cytology
/ Kidney Tubules, Proximal - embryology
/ Loop of Henle - blood supply
/ Mice
/ microRNA
/ Neovascularization, Physiologic - genetics
/ Ribonuclease III - metabolism
/ Ribonuclease III - physiology
/ stroma
