Journal Article

Early Life Exposure to Fructose Alters Maternal, Fetal and Neonatal Hepatic Gene Expression and Leads to Sex-Dependent Changes in Lipid Metabolism in Rat Offspring

Bernal, Angelica, Yap, Cassandra, Clayton, Zoe E., Vickers, Mark H., Sloboda, Deborah M.

2015

Overview

Fructose consumption is associated with altered hepatic function and metabolic compromise and not surprisingly has become a focus for perinatal studies. We have previously shown that maternal fructose intake results in sex specific changes in fetal, placental and neonatal outcomes. In this follow-up study we investigated effects on maternal, fetal and neonatal hepatic fatty acid metabolism and immune modulation. Pregnant rats were randomised to either control (CON) or high-fructose (FR) diets. Fructose was given in solution and comprised 20% of total caloric intake. Blood and liver samples were collected at embryonic day 21 (E21) and postnatal day (P)10. Maternal liver samples were also collected at E21 and P10. Liver triglyceride and glycogen content was measured with standard assays. Hepatic gene expression was measured with qPCR. Maternal fructose intake during pregnancy resulted in maternal hepatic ER stress, hepatocellular injury and increased levels of genes that favour lipogenesis. These changes were associated with a reduction in the NLRP3 inflammasome. Fetuses of mothers fed a high fructose diet displayed increased hepatic fructose transporter and reduced fructokinase mRNA levels and by 10 days of postnatal age, also have hepatic ER stress, and elevated IL1β mRNA levels. At P10, FR neonates demonstrated increased hepatic triglyceride content and particularly in males, associated changes in the expression of genes regulating beta oxidation and the NLRP3 inflammasome. Further, prenatal fructose results in sex-dependant changes in levels of key clock genes. Maternal fructose intake results in age and sex-specific alterations in maternal fetal and neonatal free fatty acid metabolism, which may be associated in disruptions in core clock gene machinery. How these changes are associated with hepatic inflammatory processes is still unclear, although suppression of the hepatic inflammasome, as least in mothers and male neonates may point to impaired immune sensing.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Animals, Newborn / Body Weight - drug effects / Circadian Rhythm Signaling Peptides and Proteins - genetics / Clock gene / CLOCK protein / Embryos / Endoplasmic Reticulum Stress - drug effects / Fatty acids / Fatty Acids, Nonesterified - blood / Fatty liver / Female / Fetuses / Follow-Up Studies / Fructokinase / Fructose / Fructose - administration & dosage / Fructose - metabolism / Fructose - pharmacology / Gene expression / Gene Expression Regulation, Developmental - drug effects / Genes / Glucose / Glycogen / Glycogen - metabolism / Immunomodulation / Inflammasomes / Inflammation / Insulin resistance / Interleukin 1 / Kinases / Lipid metabolism / Lipid Metabolism - drug effects / Lipid Metabolism - genetics / Lipogenesis / Liver / Liver - embryology / Liver - growth & development / Liver - metabolism / Machinery / Machinery and equipment / Male / Males / Maternal Nutritional Physiological Phenomena / Metabolism / Neonates / Newborn babies / Obesity / Offspring / Oxidation / Placenta / Pregnancy / Prenatal Exposure Delayed Effects - genetics / Prenatal Exposure Delayed Effects - metabolism / Random Allocation / Rats / Rats, Wistar / Reverse Transcriptase Polymerase Chain Reaction / Sex / Sex Factors / Studies / Time Factors / Transporter