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Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus
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Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus
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Journal Article
Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus
2018
Overview
Enteric diseases in swine are often caused by different pathogens and thus metagenomics are a useful tool for diagnostics. The capacities of nanopore sequencing for viral diagnostics were investigated here. First, cell culture-grown porcine epidemic diarrhea virus and rotavirus A were pooled and sequenced on a MinION. Reads were already detected at 7 seconds after start of sequencing, resulting in high sequencing depths (19.2 to 103.5X) after 3 h. Next, diarrheic feces of a one-week-old piglet was analyzed. Almost all reads (99%) belonged to bacteriophages, which may have reshaped the piglet’s microbiome. Contigs matched
Bacteroides
,
Escherichia
and
Enterococcus
phages. Moreover, porcine kobuvirus was discovered in the feces for the first time in Belgium. Suckling piglets shed kobuvirus from one week of age, but an association between peak of viral shedding (10
6.42
–10
7.01
copies/swab) and diarrheic signs was not observed during a follow-up study. Retrospective analysis showed the widespread (n = 25, 56.8% positive) of genetically moderately related kobuviruses among Belgian diarrheic piglets. MinION enables rapid detection of enteric viruses. Such new methodologies will change diagnostics, but more extensive validations should be conducted. The true enteric pathogenicity of porcine kobuvirus should be questioned, while its subclinical importance cannot be excluded.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 45/23
/ 45/77
/ 45/90
/ Animals
/ Diarrhea
/ Disease
/ Feces
/ Genes
/ Genomes
/ Hogs
/ Humanities and Social Sciences
/ Kobuvirus - isolation & purification
/ Phages
/ Picornaviridae Infections - diagnosis
/ Picornaviridae Infections - transmission
/ Picornaviridae Infections - veterinary
/ Picornaviridae Infections - virology
/ Science
/ Swine
/ Swine Diseases - epidemiology
/ Swine Diseases - transmission
/ Transmissible gastroenteritis
/ Virology
/ Viruses
