Journal Article

Retreatment strategies following Small Incision Lenticule Extraction (SMILE): In vivo tissue responses

Lwin, Nyein C., Lim, Chris H. L., Riau, Andri K., Liu, Yu-Chi, Teo, Ericia P., Yam, Gary H., Mehta, Jodhbir S., Tan, Donald T.

2017

Overview

With any refractive correction, including Small Incision Lenticule Extraction (SMILE), there may be a residual refractive error that requires a retreatment. Here, we investigated the tissue responses following various retreatment procedures in a rabbit model of SMILE. All rabbits underwent a -6.00D correction with SMILE. Two weeks later, they underwent -1.00D enhancement by: (i) VisuMax Circle, followed by excimer ablation (S+C); (ii) secondary SMILE anterior to the primary procedure (S+SE); or (iii) surface ablation (S+P), and were examined for 28 days. S+P induced corneal edema and haze, and more CD11b- (23±6 cells) and TUNEL-positive (36±4 cells) cells in the central stromal superficial layers early post-operatively (p<0.001 compared to other procedures). The corneas appeared normal on day 28 after S+P, but had a lower number of keratocytes near the laser ablated plane compared to other procedures. S+SE and S+C did not induce corneal haze and resulted similar level of fibronectin. However, S+C resulted in more inflammatory (10±2 cells; p = 0.001) and apoptotic cells (25±2 cells; p<0.001) compared to S+SE (7±1 inflammatory cells and 21±3 apoptotic cells) early post-operatively. In conclusion, each SMILE retreatment method resulted in unique tissue responses. S+SE offers advantages, such as minimal inflammation and cell death, as well as maintaining a 'flap-less' surgery, over other procedures. However, depending on the degree of enhancement, the lenticule may become too thin to be extracted and the procedure becomes more difficult to perform than S+C and S+P. S+P can maintain corneal integrity by avoiding flap creation and is technically more simple to perform than the others, but the surgery needs to be supplemented with mitomycin-C in order to reduce inflammation and modulate better wound healing.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Ablation / Animals / Apoptosis / Biology and Life Sciences / Care and treatment / Cataracts / CD11b antigen / CD11b Antigen - metabolism / Cell death / Complications and side effects / Cornea / Cornea - diagnostic imaging / Cornea - metabolism / Cornea - pathology / Corneal Stroma - surgery / Edema / Engineering and Technology / Error analysis / Extraction / Fibronectin / Fibronectins - metabolism / Inflammation / Integrity / Ki-67 Antigen - metabolism / Laboratories / Laser therapy / Lasers / Lasers, Excimer / Medical schools / Medicine and Health Sciences / Microscopy, Confocal / Mitomycin C / Photorefractive Keratectomy / Rabbits / Refractive errors / Research and Analysis Methods / Risk factors / Stem cells / Surgery / Tissue engineering / Tomography / Tomography, Optical Coherence / Wound healing