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Production of monoclonal antibodies against whole virus particles of foot-and-mouth disease virus serotype O and A and their potential use in quantification of intact virus for vaccine manufacture
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Production of monoclonal antibodies against whole virus particles of foot-and-mouth disease virus serotype O and A and their potential use in quantification of intact virus for vaccine manufacture
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Production of monoclonal antibodies against whole virus particles of foot-and-mouth disease virus serotype O and A and their potential use in quantification of intact virus for vaccine manufacture
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Journal Article
Production of monoclonal antibodies against whole virus particles of foot-and-mouth disease virus serotype O and A and their potential use in quantification of intact virus for vaccine manufacture
2008
Overview
A double antibody sandwich ELISA for quantification of foot-and-mouth disease (FMD) whole virus particle was developed using two monoclonal antibodies (mAbs) against FMD virus (FMDV) serotype O and A. The antibody binding epitopes were characterized. The two mAbs reacted with all of the tested FMDV strains. The use of these two mAbs in the sandwich ELISA will allow the quantification of FMD whole virus particles during vaccine manufacture.
Publisher
Elsevier Ltd,Elsevier,Elsevier Limited
Subject
/ Animals
/ Antibodies, Monoclonal - isolation & purification
/ Antibodies, Viral - isolation & purification
/ Biological and medical sciences
/ Double antibody sandwich ELISA
/ Enzyme-Linked Immunosorbent Assay - methods
/ Female
/ Foot-and-mouth disease virus
/ Foot-and-Mouth Disease Virus - immunology
/ Fundamental and applied biological sciences. Psychology
/ Mice
/ Vaccines
/ Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
/ Virion
/ Virology
