Journal Article

Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

Holm, Jacob B, Kleiveland, Charlotte Ramstad, Derer, Stefanie, Sina, Christian, Larsen, Ida S, Sulek, Karolina, Madsen, Lise, Sonne, Si B, Rudi, Knut, Rivollier, Aymeric, Kristiansen, Karsten, Angell, Inga Leena, Marette, André, Lea, Tor Erling, Indrelid, Stine Hufthammer, Damgaard, Mads V, von Burg, Nicole, Fjære, Even, Arnoldussen, Yke Jildouw, Agace, William, Åkesson, Caroline Piercey, Treebak, Jonas Thue, Jensen, Benjamin Anderschou Holbech, Pærrgaard, Simone I, Agrinier, Anne-Laure

2021

Overview

Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/21 / 13/31 / 13/51 / 38/22 / 38/23 / 38/77 / 631/250/347 / 631/61/514/2254 / 64/60 / 692/163/2743 / 692/163/2743/137 / 692/699/1702/393 / 82/58 / Adaptive immunity / Animal models / Animals / Basic Medicine / Casein / Data points / Diet / Dietary supplements / Digestive system / Forkhead Transcription Factors - immunology / Forkhead Transcription Factors - metabolism / Foxp3 protein / Gastrointestinal system / Gastrointestinal tract / High fat diet / Homeostasis / Homeostasis - immunology / Humanities and Social Sciences / Immune response / Immune system / Immunologi inom det medicinska området (Här ingår: Cell- och immunterapi) / Immunology / Immunology in the Medical Area (including Cell and Immunotherapy) / Immunoregulation / Interleukin 17 / Interleukin-17 - immunology / Interleukin-17 - metabolism / Intestinal microflora / Intestine / Intestine, Large - immunology / Intestine, Large - metabolism / Intestine, Large - microbiology / Intestine, Small - immunology / Intestine, Small - metabolism / Intestine, Small - microbiology / Large intestine / Low fat / Low fat diet / Lymphocytes / Lymphocytes T / Lysates / Male / Medical and Health Sciences / Medicin och hälsovetenskap / Medicinska och farmaceutiska grundvetenskaper / Methylococcus capsulatus / Methylococcus capsulatus - chemistry / Methylococcus capsulatus - immunology / Mice / Mice, Inbred C57BL / Microbial activity / Microbiota / Microbiota - immunology / Microorganisms / multidisciplinary / Nuclear Receptor Subfamily 1, Group F, Member 3 - immunology / Nuclear Receptor Subfamily 1, Group F, Member 3 - metabolism / Nutrient deficiency / Obesity - immunology / Proteins - immunology / Proteins - metabolism / Science / Science (multidisciplinary) / T-Lymphocytes, Regulatory - immunology / T-Lymphocytes, Regulatory - metabolism / Thermal stress