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Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids
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Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids
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Journal Article
Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids
2014
Overview
Biomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model that mimics the 3D organization and regionalization of a microtumor region. We report that a low-temperature plasma jet, using helium flow in open air, inhibits HCT116 colon carcinoma MCTS growth in a dose-dependent manner. This growth inhibition is associated with the loss of Ki67, and the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation. This regionalized genotoxic effect leads to massive cell death and loss of the MCTS proliferative region. The use of reactive oxygen species (ROS), scavenger N-acetyl cysteine (NAC) and plasma-conditioned media demonstrate that the ROS generated in the media after exposure to low-temperature plasma play a major role in these observed effects. These findings strengthen the interest in the use of MCTS for the evaluation of antiproliferative strategies, and open new perspectives for studies dedicated to demonstrate the potential of low-temperature plasma in cancer therapy.
Publisher
IOP Publishing,Institute of Physics: Open Access Journals
Subject
/ Cancer
/ Colon
/ genotoxic and cytotoxic effects
/ Histones
/ Media
/ multi-cellular tumor spheroid
/ Physics
/ Plasmas
/ Tumors
