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Large-scale de novo DNA synthesis: technologies and applications
by
Kosuri, Sriram
, Church, George M
in
45
/ 49
/ 631/1647/1511
/ 631/1647/338/552
/ 631/92/95
/ Animals
/ Automation
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical Engineering/Biotechnology
/ Computational Biology - methods
/ Deoxyribonucleic acid
/ DNA
/ DNA - biosynthesis
/ DNA - genetics
/ DNA Barcoding, Taxonomic
/ DNA synthesis
/ Error correction
/ Gene sequencing
/ Genetic engineering
/ Genetic Engineering - methods
/ Genetic research
/ Genomics
/ High-Throughput Nucleotide Sequencing
/ Humans
/ Life Sciences
/ Methods
/ Mice
/ Molecular Biology
/ Nucleotide sequence
/ Oligonucleotide Array Sequence Analysis
/ Oligonucleotides - genetics
/ Polymers - chemistry
/ Protein Engineering - methods
/ Proteomics
/ review-article
/ Scientific method
/ Sequence Analysis, DNA
/ Synthesis
/ Synthetic biology
2014
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Large-scale de novo DNA synthesis: technologies and applications
by
Kosuri, Sriram
, Church, George M
in
45
/ 49
/ 631/1647/1511
/ 631/1647/338/552
/ 631/92/95
/ Animals
/ Automation
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical Engineering/Biotechnology
/ Computational Biology - methods
/ Deoxyribonucleic acid
/ DNA
/ DNA - biosynthesis
/ DNA - genetics
/ DNA Barcoding, Taxonomic
/ DNA synthesis
/ Error correction
/ Gene sequencing
/ Genetic engineering
/ Genetic Engineering - methods
/ Genetic research
/ Genomics
/ High-Throughput Nucleotide Sequencing
/ Humans
/ Life Sciences
/ Methods
/ Mice
/ Molecular Biology
/ Nucleotide sequence
/ Oligonucleotide Array Sequence Analysis
/ Oligonucleotides - genetics
/ Polymers - chemistry
/ Protein Engineering - methods
/ Proteomics
/ review-article
/ Scientific method
/ Sequence Analysis, DNA
/ Synthesis
/ Synthetic biology
2014
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Do you wish to request the book?
Large-scale de novo DNA synthesis: technologies and applications
by
Kosuri, Sriram
, Church, George M
in
45
/ 49
/ 631/1647/1511
/ 631/1647/338/552
/ 631/92/95
/ Animals
/ Automation
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical Engineering/Biotechnology
/ Computational Biology - methods
/ Deoxyribonucleic acid
/ DNA
/ DNA - biosynthesis
/ DNA - genetics
/ DNA Barcoding, Taxonomic
/ DNA synthesis
/ Error correction
/ Gene sequencing
/ Genetic engineering
/ Genetic Engineering - methods
/ Genetic research
/ Genomics
/ High-Throughput Nucleotide Sequencing
/ Humans
/ Life Sciences
/ Methods
/ Mice
/ Molecular Biology
/ Nucleotide sequence
/ Oligonucleotide Array Sequence Analysis
/ Oligonucleotides - genetics
/ Polymers - chemistry
/ Protein Engineering - methods
/ Proteomics
/ review-article
/ Scientific method
/ Sequence Analysis, DNA
/ Synthesis
/ Synthetic biology
2014
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Large-scale de novo DNA synthesis: technologies and applications
Journal Article
Large-scale de novo DNA synthesis: technologies and applications
2014
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Overview
This Review discusses large-scale
de novo
DNA synthesis via oligos or arrays, describes gene assembly and error correction and considers applications for large-scale DNA synthesis.
For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the
de novo
synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale
de novo
DNA constructs, as well as the challenges and opportunities that lie ahead.
Publisher
Nature Publishing Group US,Nature Publishing Group
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