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Large-scale de novo DNA synthesis: technologies and applications
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Large-scale de novo DNA synthesis: technologies and applications
Large-scale de novo DNA synthesis: technologies and applications
Journal Article

Large-scale de novo DNA synthesis: technologies and applications

2014
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Overview
This Review discusses large-scale de novo DNA synthesis via oligos or arrays, describes gene assembly and error correction and considers applications for large-scale DNA synthesis. For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.