Journal Article

Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog

Pretto-Giordano, Lucienne Garcia, de Alcântara, Brígida Kussumoto, Di Santis, Giovana Wingeter, da Nóbrega, Daniela Farias, Headley, Selwyn Arlington, Gomes, Lucas Alécio, Itano, Eiko Nakagawa, Macagnan, Rafaela, Leite, Katherine Moura, Ono, Mario Augusto, Alfieri, Amauri Alcindo

2017

Overview

Paracoccidioidomycosis (PCM) is an endemic disease of humans from Latin America that is caused by Paracoccidioides brasiliensis and P. lutzii , with most cases of PCM in domestic animals being associated with P. brasiliensis . This study presents the clinical, cytological, mycological, serological, and molecular findings associated with P. brasiliensis in a dog from Southern Brazil. Fine needle biopsies were collected from the skin and several lymph nodes of a 5-year-old female Labrador dog that had enlargement of most superficial lymph nodes. Cytology of the skin and lymph nodes revealed pyogranulomatous dermatitis and lymphadenitis associated with fine-necked, budding fungal structures consistent with the Paracoccidioides genus of organisms; mycological culture derived from the lymph node aspirate demonstrated similar budding structures. Serological assays using exoantigens obtained from the fungal culture demonstrated that the fungal organisms derived from the lymph node were antigenically similar to P. brasiliensis by immunodiffusion and Western blot. A PCR assay, using the fungal culture as input, amplified a partial segment of the internal transcribed spacer 1 and 2 regions of P. brasiliensis; direct sequencing and phylogenetic analyses confirmed the PCR product as P. brasiliensis . The combined cytological, mycological, serological, and molecular findings confirmed a diagnosis of fungal dermatitis and lymphadenitis due to P. brasiliensis in this dog. This case represents the third description of clinical PCM in dogs and the first confirmation of mycotic dermatitis associated with P. brasiliensis in this species. The participation of dogs in the possible dissemination of PCM is reviewed, and it is proposed that dogs are probable accidental hosts in the epidemiological cycle associated with P. brasiliensis .

• Publisher: Springer Netherlands,Springer,Springer Nature B.V
• Subject: Analysis / Animals / Antifungal agents / Antigens, Fungal - analysis / Apathy / Biomedical and Life Sciences / Biopsy, Fine-Needle / Brazil / Cluster Analysis / Dermatitis / Dermatitis - etiology / Dermatitis - pathology / Dermatitis - veterinary / Disease prevention / DNA, Fungal - chemistry / DNA, Fungal - genetics / DNA, Ribosomal Spacer - chemistry / DNA, Ribosomal Spacer - genetics / Dog Diseases - diagnosis / Dog Diseases - microbiology / Dog Diseases - pathology / Dogs / Epidemiology / Eukaryotic Microbiology / Female / Fungi / Hematology / Histocytochemistry / Immunoassay / Immunology / Laboratories / Life Sciences / Lymph Nodes - microbiology / Lymph Nodes - pathology / Lymphadenitis / Lymphadenitis - etiology / Lymphadenitis - pathology / Lymphadenitis - veterinary / Lymphatic system / Medical Microbiology / Microbial Ecology / Microbiological Techniques / Microbiology / Microscopy / Molecular biology / Paracoccidioides / Paracoccidioides - isolation & purification / Paracoccidioides brasiliensis / Paracoccidioidomycosis - etiology / Paracoccidioidomycosis - pathology / Paracoccidioidomycosis - veterinary / Phylogeny / Plant Sciences / Preventive medicine / Sequence Analysis, DNA / Serology / Skin - microbiology / Skin - pathology