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An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis
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An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis
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Journal Article
An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis
2012
Overview
miR828 in Arabidopsis triggers the cleavage of
Trans
-
Acting SiRNA Gene 4
(
TAS4
) transcripts and production of small interfering RNAs (
ta
-siRNAs). One siRNA,
TAS4
-siRNA81(−), targets a set of MYB transcription factors including
PAP1
,
PAP2,
and
MYB113
which regulate the anthocyanin biosynthesis pathway. Interestingly, miR828 also targets MYB113, suggesting a close relationship between these MYBs, miR828, and
TAS4
, but their evolutionary origins are unknown. We found that
PAP1
,
PAP2
, and
TAS4
expression is induced specifically by exogenous treatment with sucrose and glucose in seedlings. The induction is attenuated in abscisic acid (ABA) pathway mutants, especially in
abi3
-
1
and
abi5
-
1
for
PAP1
or
PAP2
, while no such effect is observed for
TAS4
.
PAP1
is under regulation by
TAS4
, demonstrated by the accumulation of
PAP1
transcripts and anthocyanin in
ta
-siRNA biogenesis pathway mutants.
TAS4
-siR81(−) expression is induced by physiological concentrations of Suc and Glc and in
pap1
-
D
, an activation-tagged line, indicating a feedback regulatory loop exists between
PAP1
and
TAS4
. Bioinformatic analysis revealed
MIR828
homologues in dicots and gymnosperms, but only in one basal monocot, whereas
TAS4
is only found in dicots. Consistent with this observation,
PAP1
,
PAP2
, and
MYB113
dicot paralogs show peptide and nucleotide footprints for the
TAS4
-siR81(−) binding site, providing evidence for purifying selection in contrast to monocots. Extended sequence similarities between
MIR828, MYB
s, and
TAS4
support an inverted duplication model for the evolution of
MIR828
from an ancestral gymnosperm
MYB
gene and subsequent formation of
TAS4
by duplication of the miR828* arm. We obtained evidence by modified 5′-RACE for a
MYB
mRNA cleavage product guided by miR828 in
Pinus resinosa
. Taken together, our results suggest that regulation of anthocyanin biosynthesis by
TAS4
and miR828 in higher plants is evolutionarily significant and consistent with the evolution of
TAS4
since the dicot—monocot divergence.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Biomedical and Life Sciences
/ Carbohydrates - pharmacology
/ Feedback, Physiological - drug effects
/ Gene Expression Regulation, Plant
/ Gene Expression Regulation, Plant - drug effects
/ genes
/ genetics
/ glucose
/ mutants
/ Pancreatitis-Associated Proteins
/ Pinus
/ Reverse Transcriptase Polymerase Chain Reaction
/ RNA, Small Interfering - genetics
/ Sequence Homology, Amino Acid
/ Sequence Homology, Nucleic Acid
/ sucrose
/ Sugar
/ Taxus
