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Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
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Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
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Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
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Journal Article
Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
2021
Overview
Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53
+
cells (by 40.2%) induced by exposure to H
2
O
2
(60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53
+
cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of H
2
O
2
: the number of p53
+
cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE.
Publisher
Springer US,Springer,Springer Nature B.V
Subject
/ Animals
/ Arginine
/ Biomedical and Life Sciences
/ Cytoprotection - drug effects
/ Enkephalin, Leucine - analogs & derivatives
/ Enkephalin, Leucine - pharmacology
/ Glycine
/ Hydrogen Peroxide - pharmacology
/ NG-Nitroarginine methyl ester
/ NG-Nitroarginine Methyl Ester - pharmacology
/ Nucleoli
/ Oxidative Stress - drug effects
/ Peptides
/ Protective Agents - pharmacology
/ Rats
