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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

by Wagt, Sander , Falck, David , Mohaupt, Pablo , Filius, Mike , Nouta, Jan , Wuhrer, Manfred , Lageveen-Kammeijer, Guinevere S. M. , de Haan, Noortje

in 101/58 / 631/1647/296 / 631/45/1268 / 631/45/221 / 631/45/72 / 82/29 / 82/58 / Biological properties / Biological research / Biological samples / Biomarkers / Blood Proteins - chemistry / Capillary electrophoresis / Electrophoresis / Electrophoresis, Capillary - methods / Glycomics - methods / Glycosylation / Humanities and Social Sciences / Humans / Ionization / Ions / Isomerism / Isomers / Labeling / Mass spectrometry / Mass spectroscopy / multidisciplinary / N-glycans / Polysaccharides / Polysaccharides - analysis / Polysaccharides - chemistry / Polysaccharides - isolation & purification / Science / Science (multidisciplinary) / Sensitivity analysis / Sialic acids / Sialic Acids - chemistry / Spectrometry, Mass, Electrospray Ionization - methods / Tandem Mass Spectrometry - methods

2019

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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

by Wagt, Sander , Falck, David , Mohaupt, Pablo , Filius, Mike , Nouta, Jan , Wuhrer, Manfred , Lageveen-Kammeijer, Guinevere S. M. , de Haan, Noortje

2019

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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

by Wagt, Sander , Falck, David , Mohaupt, Pablo , Filius, Mike , Nouta, Jan , Wuhrer, Manfred , Lageveen-Kammeijer, Guinevere S. M. , de Haan, Noortje

2019

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Journal Article

Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

Wagt, Sander,

Falck, David,

Mohaupt, Pablo,

Filius, Mike,

Nouta, Jan,

Wuhrer, Manfred,

Lageveen-Kammeijer, Guinevere S. M.,

de Haan, Noortje

2019

Overview

The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N -glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts. In-depth characterization of complex glycomes is complicated by the immense structural diversity of glycans. Here, the authors present a mass spectrometry-based strategy for untargeted, sensitive glycan profiling and identify 167 N -glycan compositions in total human plasma.

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Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio

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