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Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis
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Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis
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Journal Article
Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis
2021
Overview
In most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one ‘obligatory crossover’ and crossovers are prevented from occurring near one another by ‘crossover interference’. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigate crossover positioning in the
Arabidopsis thaliana
wild-type, an over-expressor of the conserved E3 ligase HEI10, and a
hei10
heterozygous line. We show that crossover positions can be explained by a predictive, diffusion-mediated coarsening model, in which large, approximately evenly-spaced HEI10 foci grow at the expense of smaller, closely-spaced clusters. We propose this coarsening process explains many aspects of
Arabidopsis
crossover positioning, including crossover interference. Consistent with this model, we also demonstrate that crossover positioning can be predictably modified in vivo simply by altering HEI10 dosage, with higher and lower dosage leading to weaker and stronger crossover interference, respectively. As HEI10 is a conserved member of the RING finger protein family that functions in the interference-sensitive pathway for crossover formation, we anticipate that similar mechanisms may regulate crossover positioning in diverse eukaryotes.
Crossover numbers and positions are tightly controlled but the mechanism involved is still obscure. Here, the authors, using quantitative super-resolution cytogenetics and mathematical modelling, show that diffusion mediated coarsening of HEI10, an E3-ligase domain containing protein, may explain meiotic crossover positioning in Arabidopsis.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Chromosomal Proteins, Non-Histone - genetics
/ Chromosomal Proteins, Non-Histone - metabolism
/ Chromosomes, Plant - genetics
/ Chromosomes, Plant - metabolism
/ Crossing Over, Genetic - genetics
/ Dosage
/ Humanities and Social Sciences
/ Meiosis
/ Proteins
/ Science
/ Synaptonemal Complex - genetics
