Journal Article

The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency

Dong, Guojun, Hu, Jiang, Wang, Danying, Xu, Guohua, Zhang, Guangheng, Ruan, Banpu, Wang, Yufeng, Qian, Qian, Shang, Lianguang, Chen, Guang, Jiang, Hongzhen, Harberd, Nicholas P., Zhu, Li, Yang, Shenglong, Zhang, Anpeng, Gao, Zhenyu, Liu, Chaolei, Zeng, Dali, Guo, Longbiao, Teng, Sheng

2019

Overview

The indica and japonica rice ( Oryza sativa ) subspecies differ in nitrate (NO 3 − ) assimilation capacity and nitrogen (N) use efficiency (NUE). Here, we show that a major component of this difference is conferred by allelic variation at OsNR2 , a gene encoding a NADH/NADPH-dependent NO 3 − reductase (NR). Selection-driven allelic divergence has resulted in variant indica and japonica OsNR2 alleles encoding structurally distinct OsNR2 proteins, with indica OsNR2 exhibiting greater NR activity. Indica OsNR2 also promotes NO 3 − uptake via feed-forward interaction with OsNRT1.1B , a gene encoding a NO 3 − uptake transporter. These properties enable indica OsNR2 to confer increased effective tiller number, grain yield and NUE on japonica rice, effects enhanced by interaction with an additionally introgressed indica OsNRT1.1B allele. In consequence, indica OsNR2 provides an important breeding resource for the sustainable increases in japonica rice yields necessary for future global food security. Indica rice has higher nitrate assimilation and nitrogen use efficiency (NUE) than japonica rice, but the mechanism is unclear. Here, the authors reveal that the difference is partly due to allelic variation of a nitrate reductase encoding gene and this indica allele can increase yield potential and NUE.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 38/22 / 38/23 / 38/44 / 38/88 / 38/89 / 42 / 45 / 45/77 / 631/208/480 / 631/449/1870 / 631/449/2491/2046 / 631/61/447/8 / 82/29 / 82/80 / Alleles / Biological Transport / Crop yield / Divergence / Food security / Humanities and Social Sciences / multidisciplinary / NADPH / Nicotinamide adenine dinucleotide / Nitrate reductase / Nitrate Reductase - chemistry / Nitrate Reductase - genetics / Nitrate Reductase - metabolism / Nitrates / Nitrates - metabolism / Nitrogen / Nitrogen - metabolism / Oryza - enzymology / Oryza - genetics / Oryza - growth & development / Oryza - metabolism / Oryza sativa indica / Oryza sativa japonica / Plant Proteins - chemistry / Plant Proteins - genetics / Plant Proteins - metabolism / Reductases / Rice / Science / Science (multidisciplinary)