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Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
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Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
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Journal Article
Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
2021
Overview
Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through
Caenorhabditis elegans
. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.
Optical aberrations in Structured Illumination Microscopy (SIM) can lead to loss of resolution and artifacts making it unsuitable for thick samples. Here the authors combine Adaptive Optics and SIM (AO-3DSIM) to improve the 3D resolution and reduce artifacts, performing 3D-SIM in
C.elegans
.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Animals
/ Humanities and Social Sciences
/ Imaging
/ Imaging, Three-Dimensional - instrumentation
/ Imaging, Three-Dimensional - methods
/ Intravital Microscopy - instrumentation
/ Intravital Microscopy - methods
/ Mice
/ Microscopy, Confocal - instrumentation
/ Microscopy, Confocal - methods
/ Microscopy, Fluorescence - instrumentation
/ Microscopy, Fluorescence - methods
/ Optics
/ Science
/ Uranus
