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Highly structured homolog pairing reflects functional organization of the Drosophila genome
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Highly structured homolog pairing reflects functional organization of the Drosophila genome
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Journal Article
Highly structured homolog pairing reflects functional organization of the Drosophila genome
2019
Overview
Trans
-homolog interactions have been studied extensively in
Drosophila
, where homologs are paired in somatic cells and transvection is prevalent. Nevertheless, the detailed structure of pairing and its functional impact have not been thoroughly investigated. Accordingly, we generated a diploid cell line from divergent parents and applied haplotype-resolved Hi-C, showing that homologs pair with varying precision genome-wide, in addition to establishing
trans
-homolog domains and compartments. We also elucidate the structure of pairing with unprecedented detail, observing significant variation across the genome and revealing at least two forms of pairing: tight pairing, spanning contiguous small domains, and loose pairing, consisting of single larger domains. Strikingly, active genomic regions (A-type compartments, active chromatin, expressed genes) correlated with tight pairing, suggesting that pairing has a functional implication genome-wide. Finally, using RNAi and haplotype-resolved Hi-C, we show that disruption of pairing-promoting factors results in global changes in pairing, including the disruption of some interaction peaks.
Trans
-homolog interactions, such as homolog pairing, are highly structured and associated with gene function in
Drosophila
cells. Here, the authors use haplotype-resolved Hi-C to identify genome-wide
trans
-homolog interactions in a
Drosophila
hybrid cell line and investigate their patterns and functional roles.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/89
/ 45/22
/ 45/23
/ 45/71
/ 45/77
/ 45/88
/ 45/91
/ 631/114
/ 631/337
/ Animals
/ Chromosomes, Insect - genetics
/ Domains
/ Drosophila melanogaster - genetics
/ Drosophila Proteins - genetics
/ Female
/ Genomes
/ High-Throughput Nucleotide Sequencing
/ Homology
/ Humanities and Social Sciences
/ Insects
/ Male
/ Science
