Journal Article

Improved co-registration of ex-vivo and in-vivo cardiovascular magnetic resonance images using heart-specific flexible 3D printed acrylic scaffold combined with non-rigid registration

Roujol, Sébastien, O’Neill, Mark, Razavi, Reza, Chubb, Henry, Tschabrunn, Cory, Mukherjee, Rahul K., Anter, Elad, Rhode, Kawal, King, Andrew, Whitaker, John, Niederer, Steven, Connolly, Adam, Bishop, Martin J., Nezafat, Reza, Neji, Radhouene, Byrne, Nicholas, Puyol-Antón, Esther, Razeghi, Orod, O’Neill, Louisa, Williams, Steven E.

2019

Overview

Background Ex-vivo cardiovascular magnetic resonance (CMR) imaging has played an important role in the validation of in-vivo CMR characterization of pathological processes. However, comparison between in-vivo and ex-vivo imaging remains challenging due to shape changes occurring between the two states, which may be non-uniform across the diseased heart. A novel two-step process to facilitate registration between ex-vivo and in-vivo CMR was developed and evaluated in a porcine model of chronic myocardial infarction (MI). Methods Seven weeks after ischemia-reperfusion MI, 12 swine underwent in-vivo CMR imaging with late gadolinium enhancement followed by ex-vivo CMR 1 week later. Five animals comprised the control group, in which ex-vivo imaging was undertaken without any support in the LV cavity, 7 animals comprised the experimental group, in which a two-step registration optimization process was undertaken. The first step involved a heart specific flexible 3D printed scaffold generated from in-vivo CMR, which was used to maintain left ventricular (LV) shape during ex-vivo imaging. In the second step, a non-rigid co-registration algorithm was applied to align in-vivo and ex-vivo data. Tissue dimension changes between in-vivo and ex-vivo imaging were compared between the experimental and control group. In the experimental group, tissue compartment volumes and thickness were compared between in-vivo and ex-vivo data before and after non-rigid registration. The effectiveness of the alignment was assessed quantitatively using the DICE similarity coefficient. Results LV cavity volume changed more in the control group (ratio of cavity volume between ex-vivo and in-vivo imaging in control and experimental group 0.14 vs 0.56, p  < 0.0001) and there was a significantly greater change in the short axis dimensions in the control group (ratio of short axis dimensions in control and experimental group 0.38 vs 0.79, p  < 0.001). In the experimental group, prior to non-rigid co-registration the LV cavity contracted isotropically in the ex-vivo condition by less than 20% in each dimension. There was a significant proportional change in tissue thickness in the healthy myocardium (change = 29 ± 21%), but not in dense scar (change = − 2 ± 2%, p  = 0.034). Following the non-rigid co-registration step of the process, the DICE similarity coefficients for the myocardium, LV cavity and scar were 0.93 (±0.02), 0.89 (±0.01) and 0.77 (±0.07) respectively and the myocardial tissue and LV cavity volumes had a ratio of 1.03 and 1.00 respectively. Conclusions The pattern of the morphological changes seen between the in-vivo and the ex-vivo LV differs between scar and healthy myocardium. A 3D printed flexible scaffold based on the in-vivo shape of the LV cavity is an effective strategy to minimize morphological changes in the ex-vivo LV. The subsequent non-rigid registration step further improved the co-registration and local comparison between in-vivo and ex-vivo data.

• Publisher: BioMed Central,BioMed Central Ltd,Elsevier
• Subject: 3D printing / Algorithms / Angiology / Animals / Cardiology / Cardiovascular diseases / Chronic Disease / Co-registration / Diagnosis / Disease Models, Animal / Ex-vivo CMR / Gadolinium / Heart attack / Imaging / Ischemia / Magnetic Resonance Imaging / Medical research / Medicine / Medicine & Public Health / Models, Anatomic / Models, Cardiovascular / Myocardial Infarction - diagnostic imaging / Myocardial Infarction - pathology / Myocardial Infarction - physiopathology / Myocardial Reperfusion Injury - diagnostic imaging / Myocardial Reperfusion Injury - pathology / Myocardial Reperfusion Injury - physiopathology / Myocardium - pathology / Novels / Pliability / Predictive Value of Tests / Printing, Three-Dimensional / Radiology / Rare earth metal compounds / Reproducibility of Results / Scar imaging / Sus scrofa / Technical Notes / Ventricular Function, Left / Ventricular Remodeling