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Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
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Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
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Journal Article
Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
2017
Overview
Hybrids between divergent populations commonly show hybrid sterility; this reproductive barrier hinders hybrid breeding of the
japonica
and
indica
rice (
Oryza sativa
L.) subspecies. Here we show that structural changes and copy number variation at the
Sc
locus confer
japonica–indica
hybrid male sterility. The
japonica
allele,
Sc-j
, contains a pollen-essential gene encoding a DUF1618-domain protein; the
indica
allele,
Sc-i
, contains two or three tandem-duplicated ~ 28-kb segments, each carrying an
Sc-j
-homolog with a distinct promoter. In
Sc-j
/
Sc-i
hybrids, the high-expression of
Sc-i
in sporophytic cells causes suppression of
Sc-j
expression in pollen and selective abortion of
Sc-j
-pollen, leading to transmission ratio distortion. Knocking out one or two of the three
Sc-i
copies by CRISPR/Cas9 rescues
Sc-j
expression and male fertility. Our results reveal the gene dosage-dependent allelic suppression as a mechanism of hybrid incompatibility, and provide an effective approach to overcome the reproductive barrier for hybrid breeding.
A reproductive barrier between
japonica
and
indica
rice subspecies hinders hybrid breeding. Here, the authors reveal that structural and copy number variations of a pollen-essential gene, which encodes a DUF1618-domain protein, result in allelic suppression and subsequently cause hybrid male sterility.
