Asset Details
Do you wish to reserve the book?
Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory
Do you wish to request the book?
Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory
2019
Overview
Functional changes in spatial genome organization during human development are poorly understood. Here we report a comprehensive profile of nuclear dynamics during human cardiogenesis from pluripotent stem cells by integrating Hi-C, RNA-seq and ATAC-seq. While chromatin accessibility and gene expression show complex on/off dynamics, large-scale genome architecture changes are mostly unidirectional. Many large cardiac genes transition from a repressive to an active compartment during differentiation, coincident with upregulation. We identify a network of such gene loci that increase their association inter-chromosomally, and are targets of the muscle-specific splicing factor RBM20. Genome editing studies show that
TTN
pre-mRNA, the main RBM20-regulated transcript in the heart, nucleates RBM20 foci that drive spatial proximity between the
TTN
locus and other inter-chromosomal RBM20 targets such as
CACNA1C
and
CAMK2D
. This mechanism promotes RBM20-dependent alternative splicing of the resulting transcripts, indicating the existence of a cardiac-specific
trans
-interacting chromatin domain (TID) functioning as a splicing factory.
The spatial organization of the genome plays an important but unclearly defined role in gene regulation. Here, the authors integrate Hi-C, RNA-seq and ATAC-seq data to map cardiogenesis from pluripotent stem cells and describe an RBM20-dependent splicing factory assembling the TTN locus with other RBM20 targets.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/106
/ 14/19
/ 38/23
/ 38/32
/ 45/41
/ 49/31
/ 49/39
/ 49/88
/ 82/80
/ Cell Differentiation - genetics
/ Gene Expression Regulation, Developmental
/ Genomes
/ Heart
/ Heart - growth & development
/ Humanities and Social Sciences
/ Humans
/ Loci
/ Muscles
/ RNA
/ RNA-Binding Proteins - genetics
/ RNA-Binding Proteins - metabolism
/ RNA-Binding Proteins - physiology
/ Science
/ Splicing
