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Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery
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Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery
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Journal Article
Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery
2023
Overview
Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium
Vibrio natriegens
has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic
V. natriegens
strain leads to the production of 29.0 g · L
−1
indigoidine and consumption of 165.3 g · L
−1
formate within 72 h, achieving a formate consumption rate of 2.3 g · L
−1
· h
−1
. This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery.
Formic acid (FA) is a promising CO2-equivalent feedstock for onecarbon biorefinery, but microbial host that can efficiently utilize FA is unavailable. Here, the authors engineer a non-native closed loop in
Vibrio natriegens
and demonstrate its application in promoting FA utilization.
