Journal Article

Impact of Administered Bifidobacterium on Murine Host Fatty Acid Composition

Quigley, Eamonn, Dinan, Timothy G., Ross, R. Paul, Stanton, Catherine, Shanahan, Fergus, O’Mahony, Liam, Wall, Rebecca, Kiely, Barry, Fitzgerald, Gerald

2010

Overview

Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, α-linolenic acid, on host fatty acid composition. α-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 10⁹ microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of α-linolenic acid resulted in higher (P < 0.05) α-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive α-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with α-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with α-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.

• Publisher: Berlin/Heidelberg : Springer-Verlag,Springer-Verlag,Springer‐Verlag,Springer Nature B.V
• Subject: Adipose tissue / Administration, Oral / alpha-linolenic acid / alpha-Linolenic Acid - administration & dosage / alpha-Linolenic Acid - chemistry / alpha-Linolenic Acid - metabolism / Animals / Arachidonic acid / Bifidobacteria / Bifidobacterium / Bifidobacterium breve / Biomedical and Life Sciences / Brain / Brain Chemistry / Commensals / Dietary supplements / Docosahexaenoic acid / Eicosapentaenoic acid / Fatty acid composition / Fatty acids / Fatty Acids - chemistry / Feces - microbiology / Female / gas chromatography / Gas liquid chromatography / gastrointestinal system / Gastrointestinal tract / Inflammation / Life Sciences / Lipidology / Lipids / Liquid chromatography / Liver / Liver - chemistry / Liver - metabolism / Medical Biochemistry / Medicinal Chemistry / Mice / Mice, Inbred BALB C / Microbial Genetics and Genomics / Microbiota / Microorganisms / Neurochemistry / Nutrition / Omega-3 fatty acids / Original Article / Probiotics / therapeutics