Journal Article

Gut microbiota-derived acetate attenuates lung injury induced by influenza infection via protecting airway tight junctions

Zhang, Da-Wei, Yang, Chun, Sun, Li, Fei, Guang-He, Wu, Hui-Mei, Wei, Yuan-Yuan, Hu, Lei, Yang, Ming-Ming

2024

Overview

Background Gut microbiota (GM) have been implicated as important regulators of gastrointestinal symptom which is commonly occurred along with respiratory influenza A virus (IAV) infection, suggesting the involvement of the gut-to-lung axis in a host’s response to IAV. IAV primarily destroys airway epithelium tight junctions (TJs) and consequently causes acute respiratory disease syndrome. It is known that GM and their metabolism produce an anti-influenza effect, but their role in IAV-induced airway epithelial integrity remains unknown. Methods A mouse model of IAV infection was established. GM were analyzed using 16S rRNA gene sequencing, and short-chain fatty acids (SCFAs) levels were measured. GM depletion and fecal microbiota transplantation (FMT) were conducted to validate the role of GM in IAV infection. A pair-feeding experiment was conducted to reveal whether IAV-induced GM dysbiosis is attributed to impaired food intake. Furthermore, human bronchial epithelial (HBE) cells were cocultured with IAV in the presence or absence of acetate. TJs function was analyzed by paracellular permeability and transepithelial electronic resistance (TEER). The mechanism of how acetate affects TJs integrity was evaluated in HBE cells transfected with G protein-coupled receptor 43 (GPR43) short hairpin RNA (shRNA). Results IAV-infected mice exhibited lower relative abundance of acetate-producing bacteria (Bacteroides, Bifidobacterium, and Akkermansia) and decreased acetate levels in gut and serum. These changes were partly caused by a decrease in food consumption (due to anorexia). GM depletion exacerbated and FMT restored IAV-induced lung inflammatory injury. IAV infection suppressed expressions of TJs (occludin, ZO-1) leading to disrupted airway epithelial barrier function as evidenced by decreased TEER and increased permeability. Acetate pretreatment activated GPR43, partially restored IAV-induced airway epithelial barrier function, and reduced inflammatory cytokines levels (TNF-α, IL-6, and IL-1β). Such protective effects of acetate were absent in HBE cells transfected with GPR43 shRNA. Acetate and GPR43 improved TJs in an AMP-activated protein kinase (AMPK)-dependent manner. Conclusion Collectively, our results demonstrated that GM protected airway TJs by modulating GPR43-AMPK signaling in IAV-induced lung injury. Therefore, improving GM dysbiosis may be a potential therapeutic target for patients with IAV infection.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Acetate / Acetates / Acetates - metabolism / Analysis / Animals / Biomedical and Life Sciences / Biomedicine / Care and treatment / Composition / Diagnosis / Dysbiosis / Epithelial Cells - metabolism / Fatty acids / Fatty Acids, Volatile - metabolism / Fecal Microbiota Transplantation / G proteins / Gastrointestinal Microbiome - drug effects / Gut microbiota / Gut-lung axis / Health aspects / Humans / ImmunoVirology and ImmunoOncology / Influenza / Influenza A virus / Lung diseases / Lung Injury / Medicine/Public Health / Membrane proteins / Metronidazole / Mice / Mice, Inbred C57BL / Microbiota (Symbiotic organisms) / Neomycin / Orthomyxoviridae Infections - complications / Permeability / Protein kinases / Receptors, G-Protein-Coupled - metabolism / Risk factors / RNA / Tight junctions / Tight Junctions - metabolism