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Base editing with a Cpf1–cytidine deaminase fusion
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Journal Article
Base editing with a Cpf1–cytidine deaminase fusion
2018
Overview
A new fusion protein enables precise editing of single bases in A/T-rich regions of the human genome.
The targeting range of CRISPR–Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a CRISPR–Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of
Lachnospiraceae bacterium
Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
