Journal Article

Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles

Kim, Yeon Ju, Yang, Deok-Chun, Wang, Chao, Singh, Hina, Farh, Mohamed El-Agamy, Hwang, Kyu Hyon, Singh, Priyanka

2015

Overview

In the present study, the strain Brevibacterium frigoritolerans DC2 was explored for the efficient and extracellular synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. In addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. The dynamic light scattering results indicated the average particle size of the product was 97 nm with a 0.191 polydispersity index. Furthermore, the product was analyzed by energy dispersive X-ray spectroscopy, X-ray diffraction, and elemental mapping, which displayed the presence of elemental silver in the product. Moreover, on a medical platform, the product was checked against pathogenic microorganisms including Vibrio parahaemolyticus, Salmonella enterica, Bacillus anthracis, Bacillus cereus, Escherichia coli, and Candida albicans. The nanoparticles demonstrated antimicrobial activity against all of these pathogenic microorganisms. Additionally, the silver nanoparticles were evaluated for their combined effects with the commercial antibiotics lincomycin, oleandomycin, vancomycin, novobiocin, penicillin G, and rifampicin against these pathogenic microorganisms. These results indicated that the combination of antibiotics with biosynthesized silver nanoparticles enhanced the antimicrobial effects of antibiotics. Therefore, the current study is a demonstration of an efficient biological synthesis of silver nanoparticles by B. frigoritolerans DC2 and its effect on the enhancement of the antimicrobial efficacy of well-known commercial antibiotics.

• Publisher: Dove Medical Press Limited,Taylor & Francis Ltd,Dove Press,Dove Medical Press
• Subject: Anti-Infective Agents - chemistry / Anti-Infective Agents - metabolism / Anti-Infective Agents - pharmacology / Antibacterial agents / Antibiotics / antimicrobial activity / Antimicrobial agents / Bacillus anthracis / Bacillus cereus / Bacteria - drug effects / Biosynthesis / Biotechnology / Brevibacterium / Brevibacterium - metabolism / Brevibacterium frigoritolerans / Candida - drug effects / Candida albicans / Chemical properties / Deoxyribonucleic acid / DNA / Drug resistance / E coli / Escherichia coli / Glycerol / Gold / Medical research / Metal Nanoparticles - chemistry / Methods / Microorganisms / Nanoparticles / Nanotechnology / Original Research / Particle Size / Pathogens / Physiological aspects / Product development / Salmonella enterica / Silver / Silver - chemistry / Silver - metabolism / Silver - pharmacology / silver nanoparticles / Spectrum analysis / synergistic effect / Vibrio parahaemolyticus