Journal Article

Hypoxia sensing by hepatic stellate cells leads to VEGF-dependent angiogenesis and may contribute to accelerated liver regeneration

Beck Schimmer, Beatrice, Flury-Frei, Renata, Roth Z’graggen, Birgit, Parent, Romain, Bartosch, Birke, Kurtcuoglu, Vartan, Booy, Christa, de Zélicourt, Diane, Wenger, Roland H, Schadde, Erik, Spahn, Donat R., Dirscherl, Konstantin, Schläpfer, Martin, Fatzer, Rita, Aloman, Costica

2020

Overview

Portal vein ligation (PVL) induces liver growth prior to resection. Associating liver partition and portal vein ligation (PVL plus transection=ALPPS) or the addition of the prolyl-hydroxylase inhibitor dimethyloxalylglycine (DMOG) to PVL both accelerate growth via stabilization of HIF-α subunits. This study aims at clarifying the crosstalk of hepatocytes (HC), hepatic stellate cells (HSC) and liver sinusoidal endothelial cells (LSEC) in accelerated liver growth. In vivo , liver volume, HC proliferation, vascular density and HSC activation were assessed in PVL, ALPPS, PVL+DMOG and DMOG alone. Proliferation of HC, HSC and LSEC was determined under DMOG in vitro . Conditioned media experiments of DMOG-exposed cells were performed. ALPPS and PVL+DMOG accelerated liver growth and HC proliferation in comparison to PVL. DMOG alone did not induce HC proliferation, but led to increased vascular density, which was also observed in ALPPS and PVL+DMOG. Activated HSC were detected in ALPPS, PVL+DMOG and DMOG, again not in PVL. In vitro , DMOG had no proliferative effect on HC, but conditioned supernatant of DMOG-treated HSC induced VEGF-dependent proliferation of LSEC. Transcriptome analysis confirmed activation of proangiogenic factors in hypoxic HSC. Hypoxia signaling in HSC induces VEGF-dependent angiogenesis. HSC play a crucial role in the cellular crosstalk of rapid liver regeneration.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13 / 13/1 / 13/51 / 14/34 / 14/56 / 38/91 / 631/80/304 / 64 / 692/4020/4021/288/2140 / Angiogenesis / Animals / Biomarkers / Cell Proliferation / Cellular Biology / Disease Susceptibility / Endothelial cells / Gene expression / Hepatic Stellate Cells - metabolism / Hepatocytes / Human health and pathology / Humanities and Social Sciences / Hydroxylase / Hypoxia / Hypoxia - genetics / Hypoxia - metabolism / Hépatology and Gastroenterology / Life Sciences / Liver / Liver Regeneration / Models, Animal / Models, Biological / multidisciplinary / Neovascularization, Pathologic - genetics / Neovascularization, Pathologic - metabolism / Portal vein / Rats / Science / Science (multidisciplinary) / Stellate cells / Vascular endothelial growth factor / Vascular Endothelial Growth Factor A - genetics / Vascular Endothelial Growth Factor A - metabolism