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Characterization of MAGEG2 with testis-specific expression in mice
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Characterization of MAGEG2 with testis-specific expression in mice
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Journal Article
Characterization of MAGEG2 with testis-specific expression in mice
2017
Overview
Male germ cell development is a well-defined process occurring in numerous seminiferous tubules of the testis. Uncovering testicular novel genes related to intrinsic regulation of spermatogenesis is essential for the understanding of spermatogenesis. In the present study, we investigated mouse Mageg2, which belongs to a group of melanoma-associated antigens （MAGEs）. Mageg2 is transcribed in the testis specifically, and its expression level is increased at the pachytene spermatocyte stage, indicating that Mageg2 is expressed predominantly in germ cells. We generated an antibody against mouse MAGEG2 for further characterization at the protein level. Immunoblot analysis suggested that MAGEG2 has specific testicular expression and the expression primarily occurred in pachytene spermatocytes. Proteomic analyses demonstrated that mouse MAGEG2 binded to testicular germ cell-specific serine/threonine-protein kinase 31 （STK31） and heat shock protein 9 （HSPA9）. Direct binding with both interaction partners was confirmed by co-immunoprecipitation. We found that STK31 and HSPA9 bind MAGEG2 directly but not with each other. Interestingly, MAGEG2 reduced the kinase activity of STK31. Our study suggests that mouse MAGEG2 has at least two functions, including chaperone activity related to HSPA9 and regulation of pachytene spermatocyte-specific kinase, STK31. Altogether, our results provide the first information about MAGEG2 at the transcript and protein levels and suggest its potential molecular functions.
Publisher
Medknow Publications and Media Pvt. Ltd,Medknow Publications & Media Pvt. Ltd,Medknow Publications & Media Pvt Ltd,Wolters Kluwer Medknow Publications
Subject
/ Antigens
/ Cloning
/ DNA
/ Genes
/ heat shock proteins; serine/threonine kinase; spermatogenesis; testis
/ Heat-Shock Proteins - genetics
/ Heat-Shock Proteins - metabolism
/ Kinases
/ Male
/ Mice
/ Original
/ Protein-Serine-Threonine Kinases - genetics
/ Protein-Serine-Threonine Kinases - metabolism
/ Rodents
/ Sperm
/ Spermatogenesis - physiology
/ 免疫印迹分析
/ 小鼠睾丸
/ 特异表达
/ 睾丸生殖细胞
/ 蛋白水平
/ 表征
/ 雄性生殖细胞
