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The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages
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The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages
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Journal Article
The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages
2019
Overview
Efflux pumps are membrane protein complexes conserved in all living organisms. Beyond being involved in antibiotic extrusion in several bacteria, efflux pumps are emerging as relevant players in pathogen-host interactions. We have investigated on the possible role of the efflux pump network in
Shigella flexneri
, the etiological agent of bacillary dysentery. We have found that
S
.
flexneri
has retained 14 of the 20 pumps characterized in
Escherichia coli
and that their expression is differentially modulated during the intracellular life of
Shigella
. In particular, the
emrKY
operon, encoding an efflux pump of the Major Facilitator Superfamily, is specifically and highly induced in
Shigella
-infected U937 macrophage-like cells and is activated in response to a combination of high K
+
and acidic pH, which are sensed by the EvgS/EvgA two-component system. Notably, we show that following
S
.
flexneri
infection, macrophage cytosol undergoes a mild reduction of intracellular pH, permitting EvgA to trigger the
emrKY
activation. Finally, we present data suggesting that EmrKY is required for the survival of
Shigella
in the harsh macrophage environment, highlighting for the first time the key role of an efflux pump during the
Shigella
invasive process.
