Journal Article

Pathogens Associated with Bovine Mastitis: The Experience of Bosnia and Herzegovina

Nicholas, Robin A. J., Mutevelić, Tarik, Rifatbegović, Maid, Maksimović, Zinka, Hadžiomerović, Mithat

2024

Overview

To obtain improved insights into the complex microbial aetiology of bovine mastitis, this study investigated the pathogens involved in cattle mastitis in Bosnia and Herzegovina. A total of 179 milk samples from cows with clinical mastitis (CM) and subclinical mastitis (SCM), as well as eight bulk tank milk (BTM) samples from 48 dairy farms, were analysed by standard bacteriological and mycological methods. Mycoplasma detection and identification were performed using culture techniques and real-time polymerase chain reaction (PCR). A total of 88 (49.2%) mastitis samples were positive for known mastitis pathogens at 32 of 47 farms (68.1%). Mycoplasma bovis was a predominant pathogen (25/187; 13.4%) in the majority of herds (14/48; 29.2%) and accounted for 48.9% of positive CM samples. Escherichia coli was the second most dominant CM pathogen (34%), followed by Streptococcus agalactiae (10.6%), whereas Staphylococcus aureus and coagulase-negative staphylococci were the most common in SCM samples (17.1%). Other mastitis pathogens included Candida spp. and Prototheca zopfii. Two BTM samples were positive for M. bovis only, and one was positive for a mixed culture of S. aureus and Streptococcus uberis. The finding of various causative agents of bovine mastitis, with M. bovis emerging as the main pathogen, emphasizes the significance of comprehensive testing that includes not only common mastitis pathogens but also mycoplasmas, fungi, and algae.

• Publisher: MDPI AG,MDPI
• Subject: Algae / Analysis / Antimicrobial resistance / Bacteria / Bosnia-Herzegovina / bovine mastitis / bulk milk / Candida / Cattle / clinical and subclinical mastitis / Coagulase / Cow's milk / Dairy cattle / Dairy farming / Dairy farms / Dairy industry / Diagnosis / Drug resistance in microorganisms / Economic importance / Escherichia coli / Ethylenediaminetetraacetic acid / Etiology / Farms / Fungi / Inflammation / Lactose / Mammary gland / Mastitis / milk / Milk production / Mixed culture / Morphology / Mycoplasma bovis / mycoplasmas / Pathogenic microorganisms / Pathogens / Polymerase chain reaction / Prototheca zopfii / Public health / quantitative polymerase chain reaction / Staphylococcus aureus / Staphylococcus infections / Streptococcus agalactiae / Streptococcus infections / Streptococcus uberis