Journal Article

plant apoplasm is an important recipient compartment for nematode secreted proteins

Jaubert, Stéphanie, Vieira, Paulo, Danchin, Etienne G.J, de Almeida-Engler, Janice, Crozat, Carine, Engler, Gilbert, Castagnone-Sereno, Philippe, Hussey, Richard S, Abad, Pierre, Neveu, Cédric, Rosso, Marie-Noëlle

2011

Overview

Similarly to microbial pathogens, plant-parasitic nematodes secrete into their host plants proteins that are essential to establish a functional interaction. Identifying the destination of nematode secreted proteins within plant cell compartment(s) will provide compelling clues on their molecular functions. Here the fine localization of five nematode secreted proteins was analysed throughout parasitism in Arabidopsis thaliana. An immunocytochemical method was developed that preserves both the host and the pathogen tissues, allowing the localization of nematode secreted proteins within both organisms. One secreted protein from the amphids and three secreted proteins from the subventral oesophageal glands involved in protein degradation and cell wall modification were secreted in the apoplasm during intercellular migration and to a lower extent by early sedentary stages during giant cell formation. Conversely, another protein produced by both subventral and dorsal oesophageal glands in parasitic stages accumulated profusely at the cell wall of young and mature giant cells. In addition, secretion of cell wall-modifying proteins by the vulva of adult females suggested a role in egg laying. The study shows that the plant apoplasm acts as an important destination compartment for proteins secreted during migration and during sedentary stages of the nematode.

• Publisher: Oxford University Press,Oxford University Press (OUP)
• Subject: Animals / apoplast / Arabidopsis / Arabidopsis - metabolism / Arabidopsis - parasitology / Arabidopsis thaliana / Biological and medical sciences / Botanics / Cell Wall / Cell Wall - metabolism / Cell Wall - parasitology / Cell walls / Female / females / Fundamental and applied biological sciences. Psychology / Giant cells / Helminth Proteins / Helminth Proteins - metabolism / host plants / Host-Parasite Interactions / Life Sciences / metabolism / Nematoda / Nematodes / oviposition / parasitism / parasitology / pathogens / Plant cells / Plant Diseases / Plant Diseases - parasitology / Plant glands / Plant parasitic nematodes / plant proteins / Plant Roots / Plant Roots - metabolism / Plant Roots - parasitology / Plants / protein degradation / Protein Transport / Proteins / RESEARCH PAPER / Research Papers / Secretion / Tylenchoidea / Tylenchoidea - metabolism / Vegetal Biology / vulva