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Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates
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Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates
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Journal Article
Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates
2020
Overview
Glycosaminoglycans (GAGs) are polysaccharides produced by most mammalian cells and involved in a variety of biological processes. However, due to the size and complexity of GAGs, detailed knowledge about the structure and expression of GAGs by cells, the glycosaminoglycome, is lacking. Here we report a straightforward and versatile approach for structural domain mapping of complex mixtures of GAGs, GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs to generate internal, terminating, and initiating domains, and nanoflow reversed-phase ion-pairing chromatography with negative mode higher-energy collision dissociation (HCD) tandem mass spectrometry (MS/MS) for structural characterization of the individual domains. GAGDoMa provides a detailed structural insight into the glycosaminoglycome, and offers an important tool for deciphering the complexity of GAGs in cellular physiology and pathology.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Chondroitin Sulfates - analysis
/ Chondroitin Sulfates - chemistry
/ Chromatography, High Pressure Liquid
/ Chromatography, Reverse-Phase
/ Clinical Laboratory Medicine
/ Dermatan Sulfate - chemistry
/ Enzymes
/ Glycosaminoglycans - analysis
/ Humanities and Social Sciences
/ Humans
/ Mapping
/ Science
