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Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology
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Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology
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Journal Article
Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology
2014
Overview
Intracellular targeting of mRNAs has recently emerged as a prevalent mechanism to control protein localization. For mitochondria, a cotranslational model of protein import is now proposed in parallel to the conventional posttranslational model, and mitochondrial targeting of mRNAs has been demonstrated in various organisms. Voltage-dependent anion channels (VDACs) are the most abundant proteins in the outer mitochondrial membrane and the major transport pathway for numerous metabolites. Four nucleus-encoded VDACs have been identified in Arabidopsis thaliana . Alternative cleavage and polyadenylation generate two VDAC3 mRNA isoforms differing by their 3′ UTR. By using quantitative RT-PCR and in vivo mRNA visualization approaches, the two mRNA variants were shown differentially associated with mitochondria. The longest mRNA presents a 3′ extension named alternative UTR (aUTR) that is necessary and sufficient to target VDAC3 mRNA to the mitochondrial surface. Moreover, aUTR is sufficient for the mitochondrial targeting of a reporter transcript, and can be used as a tool to target an unrelated mRNA to the mitochondrial surface. Finally, VDAC3–aUTR mRNA variant impacts mitochondria morphology and size, demonstrating the role of mRNA targeting in mitochondria biogenesis.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Biochemistry, Molecular Biology
/ Density
/ Mitochondrial Membrane Transport Proteins - genetics
/ Mitochondrial Membrane Transport Proteins - metabolism
/ Mitochondrial Membranes - metabolism
/ Mutation
/ Proteins
/ reverse transcriptase polymerase chain reaction
/ RNA
/ Voltage-Dependent Anion Channels - genetics
/ Voltage-Dependent Anion Channels - metabolism
/ Yeasts
