Journal Article

LncRNA CRNDE ameliorates bone fracture by regulating cell viability and apoptosis of osteoblasts

Han, Zhen, Huang, Yingxuan, Wei, Chengjian, Ye, Shiqi, Li, Yuanfeng

2025

Overview

Background Delayed healing is a common postoperative complication among fractured patients, imposing an additional financial burden. This research examined the clinical relationship between CRNDE and delayed fracture healing (DFH) and the potential regulatory mechanisms underlying fracture improvement. Methods qRT-PCR was utilized to assess the expression of CRNDE and miR-29a-3p in serum and cellular samples, and to evaluate the expression of genes associated with osteogenic differentiation. The diagnostic and predictive significance of serum CRNDE was analyzed using ROC analysis and logistic regression. Additionally, an hFOB 1.19 osteogenic differentiation model was established. The CCK-8 assay and flow cytometry techniques were used to investigate the effects of silencing CRNDE, as well as the concurrent inhibition of both CRNDE and miR-29a-3p, on the proliferation and apoptosis of hFOB 1.19 cells. Results CRNDE was down-regulated, while miR-29a-3p was up-regulated in DFH patients. The serum CRNDE could effectively identify DFH patients and predict the DFH occurrence. In the hFOB 1.19 osteogenic differentiation model, silencing CRNDE led to a significant decrease in the expression of osteogenic differentiation markers, a reduction in the proliferation activity of hFOB 1.19 cells, and an increase in apoptosis. There was a negative regulatory interaction between CRNDE and miR-29a-3p. Concurrently inhibiting the expression of both CRNDE and miR-29a-3p could effectively restore the functional activity of hFOB 1.19 cells. Conclusion Serum CRNDE holds potential as a biomarker for the diagnosis and prediction of DFH. The sponging effect of CRNDE on miR-29a-3p could ameliorate fracture healing.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Apoptosis / Apoptosis - genetics / Apoptosis - physiology / Biomarkers / Bone diseases / Bone fracture / Bone healing / Bone surgery / Cartilage / Cell Differentiation - genetics / Cell growth / Cell Line / Cell proliferation / Cell Proliferation - genetics / Cell Survival - genetics / Cell Survival - physiology / Cell viability / Cholecystokinin / Complications / Diagnosis / Female / Flow cytometry / Fracture Healing - genetics / Fracture Healing - physiology / Fractures / Fractures, Bone - blood / Fractures, Bone - genetics / Fractures, Bone - pathology / Genetic aspects / Hospitals / Humans / LncRNA / Male / Medicine / Medicine & Public Health / MicroRNAs / MicroRNAs - genetics / Middle Aged / MiRNA / Orthopedics / Osteoblasts / Osteoblasts - metabolism / Osteoblasts - pathology / Osteoblasts - physiology / Osteogenesis - genetics / Osteoporosis / Physiology / Postoperative / RNA, Long Noncoding - blood / RNA, Long Noncoding - genetics / RNA, Long Noncoding - physiology / Surgical Orthopedics / Traditional Chinese medicine