Asset Details
Do you wish to reserve the book?
Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
Do you wish to request the book?
Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
2011
Overview
Background
Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy.
Methods
The peptide and gene expression level of adrenomedullin in small antral follicles, large antral follicles and CL was studied by real-time RT-PCR and EIA. The effect of ADM treatment on oestradiol production in 5-day follicular culture and on progesterone production from CL of different pregnant stages was measured by EIA. The interaction of ADM and ET-1 in follicles and CL at their gene expression level was studied by real-time RT-PCR.
Results
In the rat ovary, the gene expression of
Adm
increased during development from small antral follicles to large antral follicles and CL. In vitro treatment of preantral follicular culture for 5 days with ADM increased oestradiol production but did not affect follicular growth or ovulation rate. The regulation of progesterone production by ADM in CL in culture was pregnancy-stage dependent, inhibitory at early and late pregnancy but stimulatory at mid-pregnancy, which might contribute to the high progesterone production rate of the CL at mid-pregnancy. Moreover, the interaction between ADM and ET-1 at both the production and functional levels indicates that these two vasoactive peptides may form an important local, fine-tuning regulatory system together with LH and prolactin for progesterone production in rat CL.
Conclusions
As the CL is the major source of progesterone production even after the formation of placenta in rats, ADM may be an important regulator in progesterone production to meet the requirement of pregnancy.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Adrenomedullin - pharmacology
/ Animals
/ Calcitonin Gene-Related Peptide - pharmacology
/ Chorionic Gonadotropin - metabolism
/ Corpus Luteum - drug effects
/ Corpus Luteum - growth & development
/ Female
/ Gene Expression Regulation - drug effects
/ Grants
/ Medicine
/ Ovarian Follicle - drug effects
/ Ovarian Follicle - growth & development
/ Ovarian Follicle - metabolism
/ Peptide Fragments - pharmacology
/ Peptides
/ Pregnancy Proteins - genetics
/ Pregnancy Proteins - metabolism
/ Pregnancy Proteins - secretion
/ Rats
/ Receptor Activity-Modifying Protein 2 - antagonists & inhibitors
/ Receptor Activity-Modifying Protein 2 - genetics
/ Receptor Activity-Modifying Protein 2 - metabolism
/ Receptors, Calcitonin Gene-Related Peptide - antagonists & inhibitors
/ Rodents
