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A LC-MS–based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

by Syed, Ismail , Kolar, Matthew J , Kahn, Barbara B , Chu, Qian , Homan, Edwin A , Saghatelian, Alan , Borén, Jan , Ståhlman, Marcus , Zhang, Tejia , Smith, Ulf , Chen, Shili

in 631/1647/2196/1380 / 631/1647/296 / 631/92/287 / 639/638/11/872 / Analytical Chemistry / Animals / Antidiabetics / Biological Techniques / Chromatography, Liquid - methods / Computational Biology/Bioinformatics / Diabetes mellitus / Endocrinology and Diabetes / Endokrinologi och diabetes / Esters / Fatty acids / Fatty Acids - analysis / Fatty Acids - isolation & purification / Humans / Hypoglycemic agents / Identification and classification / Inflammation / Isomers / Life Sciences / Lipids / Mass Spectrometry - methods / Measurement / Mice / Microarrays / Organic Chemistry / Palmitic acid / Product development / protocol / Saturated fatty acids / Solid phases / Time Factors / Workflow

2016

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A LC-MS–based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

by Syed, Ismail , Kolar, Matthew J , Kahn, Barbara B , Chu, Qian , Homan, Edwin A , Saghatelian, Alan , Borén, Jan , Ståhlman, Marcus , Zhang, Tejia , Smith, Ulf , Chen, Shili

2016

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A LC-MS–based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

by Syed, Ismail , Kolar, Matthew J , Kahn, Barbara B , Chu, Qian , Homan, Edwin A , Saghatelian, Alan , Borén, Jan , Ståhlman, Marcus , Zhang, Tejia , Smith, Ulf , Chen, Shili

2016

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Journal Article

A LC-MS–based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

Syed, Ismail,

Kolar, Matthew J,

Kahn, Barbara B,

Chu, Qian,

Homan, Edwin A,

Saghatelian, Alan,

Borén, Jan,

Ståhlman, Marcus,

Zhang, Tejia,

Smith, Ulf,

Chen, Shili

2016

Overview

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biological lipids. This protocol describes their extraction from serum and tissue samples, followed by enrichment and analysis by LC-MS. Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LC-MS. For a sample size of 6–12, the time frame is 2–3 d.

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Publisher

Nature Publishing Group UK,Nature Publishing Group

Subject

/ Analytical Chemistry

/ Animals

/ Antidiabetics