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Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent
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Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent
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Journal Article
Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent
2007
Overview
Non-structural phenolic compounds perform a variety of functions in plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay that utilizes Folin–Ciocalteu (F–C) reagent. The F–C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. Although the electron transfer reaction is not specific for phenolic compounds, the extraction procedure eliminates approximately 85% of ascorbic acid and other potentially interfering compounds. This assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in 1 d.
Publisher
Nature Publishing Group UK,Nature Publishing Group
