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Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

by Knauf, V.C , Knutzon, D.S. (Calgene Inc., Davis, CA) , Johnson, W.B , Thompson, G.A , Radke, S.E , Kridl, J.C

in ACEITE DE COLZA / ACIDE GRAS SATURE / ACIDOS GRASOS SATURADOS / Base Sequence / Biological and medical sciences / Biology / BIOSINTESIS / BIOSYNTHESE / Biosynthesis / Biotechnology / Brassica - genetics / Brassica - growth & development / BRASSICA CAMPESTRIS / BRASSICA NAPUS / Canola / Cloning, Molecular / DNA - genetics / Enzymes / EXPRESION GENICA / EXPRESSION DES GENES / Fatty acids / Flowers & plants / Fundamental and applied biological sciences. Psychology / Gene Expression / Genetic Engineering / Genetic technics / GENETICA MOLECULAR / Genetics / GENETIQUE MOLECULAIRE / GENIE GENETIQUE / HUILE DE COLZA / INGENIERIA GENETICA / Kanamycin - pharmacology / METABOLISME DES LIPIDES / METABOLISMO DE LIPIDOS / Methods. Procedures. Technologies / Mixed Function Oxygenases - genetics / Mixed Function Oxygenases - metabolism / Molecular Sequence Data / Oils & fats / Phenotypes / Plant Oils - metabolism / Plants / RNA, Antisense / RNA, Messenger - genetics / Seedlings / Stearates / Stearates - metabolism / Transgenic animals and transgenic plants / Transgenic plants / Unsaturated fatty acids

1992

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Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

by Knauf, V.C , Knutzon, D.S. (Calgene Inc., Davis, CA) , Johnson, W.B , Thompson, G.A , Radke, S.E , Kridl, J.C

1992

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Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

by Knauf, V.C , Knutzon, D.S. (Calgene Inc., Davis, CA) , Johnson, W.B , Thompson, G.A , Radke, S.E , Kridl, J.C

1992

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Journal Article

Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

Knauf, V.C,

Knutzon, D.S. (Calgene Inc., Davis, CA),

Johnson, W.B,

Thompson, G.A,

Radke, S.E,

Kridl, J.C

1992

Overview

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions

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Publisher

National Academy of Sciences of the United States of America,National Acad Sciences,National Academy of Sciences

Subject

ACEITE DE COLZA

/ ACIDE GRAS SATURE

/ ACIDOS GRASOS SATURADOS

/ Base Sequence

/ Biological and medical sciences

/ Biology

/ BIOSINTESIS