Journal Article

Prion strains are differentially released through the exosomal pathway

Leblanc, Pascal, Institut de génétique humaine (IGH) ; Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT), École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon, Huor, Alvina, Lehmann, Sylvain, Vilette, Didier, Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT), Raposo, Graça, Arellano Anaya, Zaïra Esperanza, Université Montpellier 1 (UM1), Mexican CONACYT; [ANR-08-MIEN-010], Institut Curie [Paris], Andréoletti, Olivier, Provansal, Monique

2015

Overview

Cell-to-cell transfer of prions is a crucial step in the spreading of prion infection through infected tissue. At the cellular level, several distinct pathways including direct cell–cell contacts and release of various types of infectious extracellular vesicles have been described that may potentially lead to infection of naïve cells. The relative contribution of these pathways and whether they may vary depending on the prion strain and/or on the infected cell type are not yet known. In this study we used a single cell type (RK13) infected with three different prion strains. We showed that in each case, most of the extracellular prions resulted from active cell secretion through the exosomal pathway. Further, quantitative analysis of secreted infectivity indicated that the proportion of prions eventually secreted was dramatically dependent on the prion strain. Our data also highlight that infectious exosomes secreted from cultured cells might represent a biologically pertinent material for spiking experiments. Also discussed is the appealing possibility that abnormal PrP from different prion strains may differentially interact with the cellular machinery to promote secretion.

• Publisher: Springer Verlag,CCSD,Springer-Verlag,Springer Basel,Springer Nature B.V
• Subject: Animals / Biochemistry / Biomedical and Life Sciences / Biomedicine / Cell Biology / Cells / Clone Cells / cultured cells / Exosomes / Exosomes - metabolism / Infections / Infectivity / Life Sciences / Mice / pathogenicity / Prion Diseases - metabolism / Prion protein / Prions / Prions - metabolism / quantitative analysis / Rabbits / Research Article / Secretion / Sheep
• ISBN: 0003503033000