Asset Details
Do you wish to reserve the book?
Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
Do you wish to request the book?
Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP
2018
Overview
GCaMP, one popular type of genetically-encoded Ca
2+
indicator, has been associated with various side-effects. Here we unveil the intrinsic problem prevailing over different versions and applications, showing that GCaMP containing CaM (calmodulin) interferes with both gating and signaling of L-type calcium channels (Ca
V
1). GCaMP acts as an impaired apoCaM and Ca
2+
/CaM, both critical to Ca
V
1, which disrupts Ca
2+
dynamics and gene expression. We then design and implement GCaMP-X, by incorporating an extra apoCaM-binding motif, effectively protecting Ca
V
1-dependent excitation–transcription coupling from perturbations. GCaMP-X resolves the problems of detrimental nuclear accumulation, acute and chronic Ca
2+
dysregulation, and aberrant transcription signaling and cell morphogenesis, while still demonstrating excellent Ca
2+
-sensing characteristics partly inherited from GCaMP. In summary, CaM/Ca
V
1 gating and signaling mechanisms are elucidated for GCaMP side-effects, while allowing the development of GCaMP-X to appropriately monitor cytosolic, submembrane or nuclear Ca
2+
, which is also expected to guide the future design of CaM-based molecular tools.
The popular genetically-encoded Ca
2+
indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca
2+
dynamics and gene expression, and develop GCaMP-X to overcome these limitations.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/19
/ 14/34
/ 14/35
/ 14/63
/ 42/109
/ 9/10
/ 9/74
/ 96/95
/ Animals
/ Biosensing Techniques - methods
/ Calcium channels (voltage-gated)
/ Calcium Channels, L-Type - metabolism
/ Fluorescent Dyes - chemistry
/ Genetic Engineering - methods
/ Green Fluorescent Proteins - chemistry
/ Green Fluorescent Proteins - genetics
/ Humanities and Social Sciences
/ Humans
/ Intravital Microscopy - methods
/ Mice
/ Neurons
/ Science
