Asset Details
Do you wish to reserve the book?
Vacuole and mitochondria patch protein Mcp1 of Saccharomyces boulardii impairs the oxidative stress response of Candida albicans by regulating 2-phenylethanol
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Vacuole and mitochondria patch protein Mcp1 of Saccharomyces boulardii impairs the oxidative stress response of Candida albicans by regulating 2-phenylethanol
Do you wish to request the book?
Vacuole and mitochondria patch protein Mcp1 of Saccharomyces boulardii impairs the oxidative stress response of Candida albicans by regulating 2-phenylethanol
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Vacuole and mitochondria patch protein Mcp1 of Saccharomyces boulardii impairs the oxidative stress response of Candida albicans by regulating 2-phenylethanol
2025
Overview
Background
Vacuole and mitochondria patch (vCLAMP) protein Mcp1 is crucial in eukaryotic cells response to environmental stress, but the mechanism of Mcp1 in
Saccharomyces boulardii
(
S. boulardii
) against pathogenic fungi is unclear.
Results
This work first explored the role of Mcp1 in
S. boulardii
against
Candida albicans
(
C. albicans
). The results showed that Mcp1 located on the vacuolar and mitochondrial membrane of
S. boulardii
. Overexpression of Mcp1 inhibited the adhesion and hyphal formation of
C. albicans
in vitro. The mice model of intestinal infection revealed that WT-
pGK1-MCP1
mutant enhanced the ability of
S. boulardii
antagonize
C. albicans
infecting gut. High performance liquid chromatography-mass spectrometry analysis demonstrated that overexpressing Mcp1 promoted the production of 2-phenylethanol. The latter is a secondary metabolite of
S. boulardii
, and can inhibit the adhesion and biofilm formation of
C. albicans
. The reverse transcription polymerase chain reaction and western blotting results confirmed Mcp1 promoted the production of 2-phenylethanol by regulating the expression level of Aro10. Notably, RNA-sequencing and Gene Ontology enrichment analyses showed that 2-phenylethanol impaired the oxidative stress response of
C. albicans
.
Conclusion
This work reveals the critical role of Mcp1 in
S. boulardii
against
C. albicans
by regulating 2-phenylethanol metabolism, which provide a theoretical basis for
S. boulardii
as antifungal biologic therapy to prevent and treat of
Candida
infection.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Animals
/ Biofilms - growth & development
/ Candida albicans - drug effects
/ Candida albicans - metabolism
/ Chemistry and Materials Science
/ Control
/ Fungal Proteins - metabolism
/ High performance liquid chromatography
/ Mcp1
/ Methods
/ Mice
/ Microbial Genetics and Genomics
/ Phenylethyl Alcohol - metabolism
/ Saccharomyces boulardii - genetics
/ Saccharomyces boulardii - metabolism
/ Vacuoles
We currently cannot retrieve any items related to this title. Kindly check back at a later time.