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Lifeact: a versatile marker to visualize F-actin
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Journal Article
Lifeact: a versatile marker to visualize F-actin
2008
Overview
Current approaches for live imaging of cellular actin dynamics have several drawbacks. Now the use of Lifeact, a 17-aa actin-binding peptide from yeast that is not present in higher eukaryotes, allows imaging of actin dynamics in live mammalian cells without disruption of function and without competition with endogenous binding proteins.
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics
in vitro
and
in vivo
and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Humans
/ Methods
/ Mice
/ Peptides
/ Rats
/ Recombinant Fusion Proteins - genetics
/ Recombinant Fusion Proteins - metabolism
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae - metabolism
